Le Phuong Chung
BIODIVERSITY AND ANTIBIOTIC ACTIVITY OF
ACTINOMYCETES ISOLATED FROM CATBA
ISLAND, VIETNAM

MASTER THESIS MAJOR BIOTECHNOLOGY
MASTER THESIS MAJOR BIOTECHNOLOGY SUPERVISORS: Dr. NGUYEN HUYNH MINH QUYEN
Dr. NGUYEN QUYNH UYEN
HANOI – 2011 LIEGE UNIVERSITY
***
VIETNAM NATIONAL UNIVERSITY, HANOI
INSTITUTE OF MICROBIOLOGY
AND BIOTECHNOLOGY
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CONTENTS
Acknowledgements i


3.3 Chromatography analyses of crude extracts of the selected strains 31
3.3.1 Thin Layer Chromatography (TLC) 31
3.3.2 High – Performance Liquid Chromatography (HPLC) 32
3.4 Primary study on cytotoxicity activity 33
3.4.1 pH-dependent color change by the selected strains 33
3.4.2 Cytotoxicity assay against human cell lines 33
3.5 Identification of the actinomycete isolates 34
3.5.1 Morphology-based identification for the Streptomyces isolates 35
3.5.2 16S rDNA sequence-based identification for the
non-Streptomyces isolates 37
Conclusion and Suggestion 38
Bibliography 40
Appendix 1 Culture media 46
Appendix 2 The 16S rDNA sequence………………………………………… …….46