V NU JO U R N A L 0 F S C IE N C E . N at . S c i . & Tech . T XIX. N.,4. 2003

STUDY ON ISOZYM ES F R O M A ED ES A E G Y P T I S T R A IN S O F S O M E
R E S ID E N T IA L A R E A S IN V IE T N A M
D o N g o e L ie n , N g u y e n T h i T h u H o a i
N a tio n a l Ư niưersity o f H a n o i
V u Duc H uong
In s titu te o f m a la ria , p a ra s ito lo g y a n d entom ology o f H a n o i
A b stra ct. lsozymes from 4 vvilcỉ- caught collections of Aedes aegypti mơsquito were
compared bv using polyacrvlamide gel electrophoresis. These collections vvere
collected from 4 residential areas of Vietnam (North, South, Centre and Central
highland area). This study revealed that there is an obvious relation between the
insecticide resistance and the expression of isozyme esterase in the mosquito. Some
differences of isozymes between larvae and adults vvere also found.
Key ivords: D erìgue fever, in se cticid e re sista n ce , s tra in , Aedes a e g y p ti%PAG E,
isozyme.
1.

In tr o d u c tio n
Dengue/ Dengue h e m o rrh a g ic fever (D F /D H F ) occurs th ro u g h o u t th e ye a r b u t

p a rtic u la rly in su m m e r tim e (u s u a lly from June to J u ly ). I t e a s ily becomes epidem ic
disease and tends to increase in S outhern region an d C e n tra l h ig h la n d o f V ie tn a m
re ce n tly. One reason fo r th is s itu a tio n is the in se cticid e resistance in dengue v iru s
vectors in w hich Aedes ae gypti is an im p o rta n t one. The m o squito has resisted to
some insecticides such as D D T. hexachloride benzen (o rg a n o c h lo rin e group ) and even
p y re th ro id group w h ic h has been considered as a p o w e rfu l g ro u p is now losing its
effect in some areas o f S o u th e rn region and C e n tra l h ig h la n d o f V ie tn a m [1, 2, 7].
T h e re vvere some dom estic and foreign researches [1 , 2 , 4, 7] s tu d y in g on the
expression o f isozym e com plex in d iffe re n t Aedes a e g y p ti s tra in s in o rd e r to c x p la in
th e re sista n ce a b ility in m o sq u ito . H ow ever, some o p in io n s are c o n tro v e rs ia l.

- C e n tra l rc iỉio n

Sonịỉ r a u (S O to w n , Phu Yen p ro vin ce ; T u y Hoa to w n (T H ),

Phu Y en: Đ on g Ha tovvn íD H ). Q ua ng T r i p rovince : T rie u Đo v illa g e

• T rie u Phong

d is tr ic t (TP ), Q ua ng T r i p rovince
- S o u th e rn rcg io n : Ben T re to w n (B T ), Ben T re p ro v in c r; D ong X oai to w n (ĐX).
B in h Phuoc p ro v in c v ; B in h ('h a n h v illa g e - Can G io d is t r ir t ( ( ’
30

The* p o l y a c r y l a m i d i * ge l is a ( i i s c o n t i n u o u s g e l w h i c h h a v e c o n c c n t r a t i o n oí 4 WI.

a n d 8% or T ‘o a n d 10°ò, size of lOcm X 8cm X 1.5 mm. Tiu* device served for
e le c tro p h o re s is is H oe fer a p p a ra tu s (S an fran cisco. U S A) o r m in iP ro te a n a p p a ra tu s

(HioKad). T he Chemicals W

•

Ỉ

S

ể fc a » «
BG

l

i

m

KRo KT NH

í

—

I

S

tề

TP ĐH



.'$1

S tu d y on isozvmes fro rn aedcs (iv ỊỊy p ti s t r a in s o f . "

F tfĩ 2: K sturaso d e te c tio n o f larva in C e n tra l re g io n and

Central h ig h la n d (on PAG ).

T h is p ic tu re is th e esterase com pariso n betw een la rv a e o f some areas in
C e n tra l re g io n and in C e n tra l h ig h la n d .
On th e side o f re g io n a l co m p a riso n . the d iffe re n c e betw een th c la r v a l E S T o f
these tvvo groups is n o t as d e a r as betw een th e a d u lt

EST discussed above.

T h e re ío rc . it can he assum eđ th a t EST e s p e cia lly EST* 1 fro m a d u lt m o s q u ito in
C e n tra l hitfhlan
3.2.

E le c tr o p h o r e t ic p r o p c r tie s o f O c ta n o l d e h y d ro g e n a s e a n d

M a la te

d e h y d ro g e n ase
Isozym e octa no l dehydrogenase o f Aedes a e g y p ti s tra in s

in some area o f

N o rth e rn , S o u th e rn , C e n tra l re gion and C e n tra l h ig h la n d has been d e tecte d b u t nơ
s ig n ific a n t d iffe re n c e Vvas found.
Isozym e

m a la te dehvdrogenase o f Aedes a e g yp ti s tra in s

in some area o f

C e n tra l rc ^ io n and C e n tra l h ig h la n d vvas also detected and th (‘ re s u lt o b ta in e d is


S tu íix 0/1 isozymes Ị r o t ì ì (ict/cs n c ịỊx p ỉi s t r a in s of..
s m iil.il tí) \vh ich S ỉiu l s II r t ;il lo u n d in Aedcs íris e ra tu s 2111(1 A r. h c n d iểrs o n ì |H| and
n o ( l i H n v n r e c n n bí* s c c n l ỉ r l v v r e n i v s i s t a n t a n d s e n s i t i v c s t n i i n s .

3.3.

E te v tro p h iằ v c tii'


IU*sitl(*s. no tliH V rrn re in th rs c tw o rn /v m e s is fo u n d betvveen head and th o ra x p a rt
and ab(lonu*n p a rt oỉ t l i r m nsquito.

4. C o n clu sio n
Ba se on t hi* a n a ly s is ()f f> isozym es fro m Aedes a e g yp ti s tra in s in d iffe re n t
rc s iíle n tia l rcg io n s o f V ie tn a m , It IS deduced th a t o n lv este rase (e s p e c ia lly KST- 1)
can 1)1* served as a goođ m a rk e r fo r in s e c tic id e re s is ta n c e « f Aedes a e g y p ti m o sq u ito .
It |> assum able due to the capacity oí’ intensive esterase activity of mosquitoes to
m srctinđc degradation, Iht;ius(» ()f the fact th a t most insecticiỉie has ester linkage
|2. 71. Hovvever, other iso/vm c analysis are also useful in explainin g the insecticide
riỉsistỉince of mosquito.

R eíeren ces
1

Tran Van Tien. Vu Sinh Nam. T rin h Quan Huan, Do Quang Ha. Truông ưyen Ninh.
I)t*n^ue íever. hemorrhagic íever and vector control in Vietnam in recent years.
Prevenũvv Mvdicint’ Journal. Vol. V III (2). 36(1998). pp: 124 (in Vietnamese).
N ntural Sciences and N ational Tochnologv Centre. Plant
Ktìvironm vntnỉ toxicology program V ỈE 197/031, 2001. (in

2

Chem istrv In s titiiti*.
ụroịvcting medicinc,
Vietnnmese).

‘t

T Chareonviriyaphnp, K. Lcrthusnee, Genetic d iffe re n tin tio n of Aedes aegypti


M.

Rui z, A.

Soca, Cr os s -

r es is t an ce to p y r e t h r o i d

and

o r g a n o p h o s p h a t e insecti ci des. sel ect ion w i t h t e m e p h o s in A e d e s a eg yp ti in Cuba. J.

A m e r ic a n M o s q u ito Control a n d Biology ỉn L a tin A m e r i c a n - a te n th s y m p o s i u m 16 (4):
20 0 0 , pp: 2 9 5 - 3 1 2 .

8.

S.H.Saul, M.fJ.Si nsko, P.R. G rim stad, G.B. Craig, Id e ntifica tion o f sibling species. Aecies
tr is e r ia tu s a n d Ae. hcindersoni bv electrophoresi s. J .M e d .E n t.V o \ 13 (6): 1 977, pp: 7 05*
708.

TAP CHÍ KHO A HOC D H Q G H N . KHTN & CN. T.X1X, N04, 2003_________

N G H IÊ N CỨU IZOZYM CỦA MỘT s ố C H Ủ N G MƯỎI A ED ES AEGYPTI
TẠI MỘT SỐ VÙNG DÂN c ư CỦA VIỆT NAM
ĐỖ N g ọ c L iê n , N g u y ễ n T h ị T h u H o à i
Đ ạ i học Quốc g ia H à N ộ i

Vủ Đửc Hương