THANH PHAN HOA HOC CUA
TINH
DAU HUONG NEP LAY
TLT
CAY
DCTA
THOM
PANDANUS
AMARYLLIA.
ROXD
- VIET NAM
Le
Thi
Anh Dao
Khoa Hoa hoc
Trutmg
DHSP - DHQG Ha Noi
1. Gidi thieu
cay
dtia
thorn cd ten khoa hoc la
Pandanus
amaryllia. Roxb thude hg
Pandanaccac.
Nd
dugc
trdng
nhi^u
b
Cau Giiy (Ha Ndi), Son Tay, Hai
Phdng

ne^p
la
e^c
la banh te; la de heo cang thom
miii
com nep, mui ben b nhiet dd
cao.
Tren
th^
gidi
da cd
nhi6u
cdng
trinh
nghien
cvax
tach
chi^t
huong nep, xac djnh thanh
phSn
hoa hgc
eiia
nd,
cung nhu
xdc
dinh
ch^t
gay
miii
huong nep [2] [3] [4] [5]. Nhung b

trieu). Trong gao
thom vdi 7
phSn
ty
eiia
2 - axetyl - 1 - pyrroline thi gao da cd mui huong
n^p.
Trong
Id
diia
thom
loai
Pandanus amaryllia. Roxb ed ham
lucmg
2 - axetyl - 1 - pyrroline cao
gip
hang tram
va hang
ng^
ISn
so vdi gao tam
th(*m.
Trong la
di'ra
Xham
ngoai chat thom ehfnh la 2 - axetyl -
1 - porroline con cd mot so chat khae gdp phan tao huong thom nep. Dd la
cdc
piperidin
ankaloit

thom trudc khi nghien
eiiu,
chiing tdi da xac dinh dugc do am theo hai phuong
phdp
va cd dd
in
tir
71-86%.
Chiing tdi xac dinh ham
lugng
tinh dau huong nep theo ba phuong
phdp.
Khi
xdc
dinh ham lugng tinh dau huong nep bang phuong phap ngam chiet vdi
ancol
etylic da
xdc
dinh dugc dieu kien tdi uu cho vice ngam
chig^t
la
ddng
ancol etylic 96" nhiet dd
ngam
chi^t
la 50°.
Y,£i
qua dugc neu tren bang 1
192
Bang 1. Ham

96" d 50" (8
gid)
3.
Ngam chiet bang ete dau
hoii
b 65 -
70°C
(8
gid)
Nhu vay ham lugng tinh dau huong ne'p tach bang
each
eat
Idi
cud'n hoi
nude
ra't thap
1,63.10
•"%.
Khi ngam chiet bang etanol, ete
dSu
hoa
thi
thu dugc nhieu hon
nhimg
ta eon chiet
them ca cac chit
phii
(elororin) nen dung dich
trd
nen xanh dam, hoi nhdt.

2
3
4
5
6
7
8
9
10
11
12
Thdi giim
km
4,11
5,35
5,83
7,09
7,6
7,8
9,52
11,52
13,20
14,37
14,63
15,58
Ten chal
etanol
axel
on
n-propanol

83,8191
193
Trong sd 12 chit thi chiing tdi da nhan
bi^t
dugc 7 chit.
DaQ
biet
cf
day chiing tdi phat
hien ra 2 - axetyl - 1 - pyrroline (pie sd 10, thdi gian
luii
14,37 phut ehiem 3,145%) la chit
thom
miii
huong
n^p
khic
vdi tinh
diu
huong
n^p
ciia
Indonexia [5] Malaixia la b chd tinh diu
huong
n^p
cua hg khdng cd chat nay ma chi cd cac ankaloit piperidin
QNQ.
Cd the do hg
khdng eit bang
Idi

CHCI3,
CHCI3/CH3OH
(3:
1),
CH3OH
thu dugc 4 phan doan
khic nhau. Trong dd phan doan 3 (CHCO3/CH3OH: 3/1) thu dugc mot giai vang cd mui huong
n£fp.
Tich
ti^p
tuc bang cac dung mdi tren eit quay dudi dung mdi chiing tdi thu dugc 2 chit
sau:
a. 3 - metyl - 2 (5H) - furanone: da xac nhan bang GC-MS, gidng vdi phd
chuin
(94%)
gdm cic manh nhu phd chuin
MS
= 98, 41, 53, 69, 83.
-
Khang
dinh bang phd hdng ngoai cung thiy triing vdi phd chuin vdi cac pic sau: 2959
cm',
1730
cm', 1581,8 em',
1464cm',
1381 cm', 1273cm',
1122
cm', 1071 cm', 1039,8 cm'
'
va742cm'

3.
Bude diu tach biet dugc 3 - metyl - 2 (5H) - furanone va 2 - axetyl - 1 - pyrroline va
nhin
dang eiu tao ciia chiing qua phuong phap khdi phd va hdng
ngoai.
(Vi khudn khd bai
vi^t
nen
chiing tdi se trinh bay cac miu chiet bang ete dau hoa amoletylic sau).
Tdi lieu tham khdo
1.
Dd Tit Lgi. Nhung cay thude va vi thude Viet Nam. NXB Khoa hgc ky thuat - 1995,
trang 1121 - 1122.
2.
Loo Choo Teng T.C.Shen and S.H. Gon. Economic BOSTANY 1979, tap 33 trang 72-
74.
194
3.
Maribel G. Nonato: The "Pandans" as patential source of new parmaceuticals".
ASOMS.
IX,
1998, Hanoi, trang 62.
4.
Ron. G. Buttery Louisa C. Long, Thomas R. Mon Quantitative Analysis of 2 - axetyl -
1 -pyrrolinee in Rice. J. Agric Food
cheml986,
tap 3, trang
112.
5.
J. Jiang: Volatile composition of pandan leaves (Pandanus - amerryllifolius). 5th -

1,62.102%
yield and was analised using GC-MS of the more than 12
components separated, 7 were
indentified.
The major components were 3 - metyl - 2 (5H) -
furanone (A) (83,8%); 2 - axetyl - 1 - pyrroline (3,145%) B; 1 - penten - 3 - ol. A, B -
compounds were isolated.
195