TỔNG LIÊN ĐOÀN LAO ĐỘNG VIỆT NAM
TRƯỜNG ĐẠI HỌC TÔN ĐỨC THẮNG
Báo cáo công trình nghiên cứu khoa học sinh viên:
KHẢO SÁT ẢNH HƯỞNG CỦA THÀNH PHẦN
MÔI TRƯỜNG VÀ NANO BẠC LÊN SỰ SINH
TRƯỞNG PHÁT TRIỂN CỦA CÂY MĂNG TÂY
(Asparagus officinalis)
GIẢNG VIÊN HƯỚNG DẪN: THS. NGUYỄN THỊ THANH HIỀN
SINH VIÊN/NHÓM SINH VIÊN THỰC HIỆN:
NGUYỄN M. CẪM TIÊN NĂM SINH: 1993 MSSV: 61103123 LỚP: 11060301
NGÔ VĂN DƯƠNG NĂM SINH: 1993 MSSV: 61103194 LỚP: 11060301
NGUYỄN P. HÙNG MẠNH NĂM SINH: 1995 MSSV: 61303627 LỚP: 13060303

Tp. Hồ Chí Minh, tháng 4 năm 2015 2

L

Trong thi gian thc hi  n Th.s
Nguyn Th Thanh Hing dng dn to mi
u kin thun l 
 t Thun, k thu
sinh h  t nhiu trong sut
th 
ng thi li cc t 
cnh o mu kin thun l c hi 

2.1.4 K thung (micropropagation) 14
c thc hin trong k thut vi ng 15
 y in vitro 16
y 16
ng 16
2.1.4.2.3 Ngu 17 4

i 18
2.1.4.2.5 S  18
 19
i ht ging. 19
2.2.2 Sn xung 20
2.c bit
c 21
n c23
ng cxanh 23
c bit c 24
 25
25
 g27
27
2.3 Ht nano bc 28
2.3.1 Gii thiu v bc kim loi 28
n ca bc 29
 n ca bc 30
2.3.4  to ht nano kim loi 31
2.3.5 ng dng ca nano bc 32

6

4.3 ng c  t mu cy chi in vitro 44
4.5 nh ng cc d  t mu cy chi in vitro 45

4.4 ng c cm 
t mu cy chi in vitro 47
4.6 ng ca nano b ng ca chi in vitro 49
4.6 ng ca nano b nhing ca chi in vitro 51
KT LUN NGH 54
PH LC 55
U THAM KHO 56
7

DANH MNG

ng ca NAA v 0; 0,5; 1,0; 1,5; 2,0 mg/l
  t mu cy chi in vitro 45

in vitro 46
ng c cm o
 mu cy chi in vitro 48
o   n vi soi ni 48
ng ca nano bc vi n 0, 2, 4, 6, 8, 10 mg/l
 ng c in vitro 49
   4 ; 6; 8 mg/l Nano bc 51
i n
in vitro 52 9 I THIU
2.4 t v
Asparagus t chi ln v
kinh t cao. c trng khp th gin gc ch yu t 
 Hu h kinh t, quan trng nh 
Asparagus officinalis L., mt loi rau cao cp
[16], [17]
.
 s dc tt c n c
c phc s dng trong vic cnh,

Nano b c s d y
Nano bc dic hu hi vi khui
bng do n
hiu sut quang hp, kht.
Xu nhu thc ti o
ng cn  n ca
 u s n mi v khoa h
cung cp mng ging tng vi nhu cu hin nay.
2.5 M
- Khng c 

- Khng ca nano b 
2.6 
ng t n c 
 ng tng sch bnh, khe
mt cao, phm cht tt cung cp cho sn xut. 11 U
c v cng
a t c v

n nh    ng th       
ng t t t ng th
n n gi
ng  a t c khnh.
T nhu v a t c
t c c v c
ghinhn  nhiu gi  Atrapoda, Begonia, Citrus, Coffea, Cymbidium, Hordeum,
Kalanchoe, Nicotiana, Panax, Ranumculus, Solanum, Oryza
ng k thugi
 c,
 Vi  ng  nhiu
 gii
[10, 11, 15, 19, 21]
.
 sinh hc thc vng
S n c sinh hc thc vt theo th 
c t nh, n k thut
bi thut cp t 
nhn nay din ra s t bc ca k thut gen
[10, 12, 15, 19, 21]
.
Nh s kt hp gic v c
nhng thuc ng mu qu. Nh 
 ca thc v t lonh
t mt t i ch trong mt thi gian ngn. S kt h
 sinh hc thc vc tin mang
li hiu qu kinh t rt l m c

[8]
:

i v
i cht  thc vt. 14


Cht auxin t y nhiu  thc v
d u khin s .
    ng dn xu         
diclophenoxyl axetic axit (2,4-ng trong s
o r
[10, 12, 22, 23]
.
NAA, IBA, 2,4-nhng auxin tng h 
i v o r.
i vi r, v n auxin c ch s n ca r, ch  n cc thp (10
-9
- 10
-12
ng ca r
-8
mg/l)
i th hing c ch.

 ra r  c li, 2,4-
  y. M 

p ca t
ng mi cht c


 th u khin  
din ra mng
[10, 12, 22, 23]
.

ng k y in vitro  dng
mt s ino acid
Abscisis acid s dng  n t c ch  bt
buc. y thc v 
ng t y nhanh s 
th c s d 
[22, 23]
.
  ng ca t  
th c         -    
 c s dng hiu qu 16

  u qu y protoplast
 m bc hi ca mui amonium (Gamborg & Shyluk, 1970; Gamborg,
1986).
t chit c h rt hiu
qu trong vic cung cp phc hp ch
sinng.
2.1.4 K thung (micropropagation)
Trong 5 thp k  k thu


bi s c bn d  
c thc hin trong k thung
[8]

Trong k thu
1984).
n 1: La chn mu cy khu.
i con hoc chi mm t mu. S i bt
 thuc s dng (Chu
u kic cung cp
t t l t t
 t ch 
 thuu gen c
    u kin tr  n h t
 ng rung.
 y in vitro
M a bt k mt k thu 
n nhi. Mt trong nh quan tr n dinh
u tin ba thp k 
phn c 18

y
c s dy in vitro nhiu loi
ng s 
c s dng ph bin nhng s 

   ng bao g  i mu   n cacbon, vitam  t
19

chi sinh s dng n BA cao cho chng cht, chi
ng hoa th hoc nhng nt nh  cui gc.
y r in vitro, s t cng hn ch kh
  sung auxin hon b sung
 thuu gen cng
[25]
.
2.1.4.2.3 Ngu
Ngun cacb c s d    ng khong 2- ng saccarose
hoc     ng ngu       
 dng. Ngu n t 
Hu hr tng hn thit cho s 
trin  i nh           
myo-inositol cc b y in vitro   sinh
 ph
[24]
.
c s dng r
    s d      Than ho 
c b ng  y in vitro.
Than hong. Than ho p
th mt s chi cho s n cc sn sinh ra
 y hoc mt s chng tit ra.
              
on, 1984). B 
 i cho vi cng

t ht c ch ra r hom m
chi sung than ho
1984).
S a h r cy in vitro u kin thit yu
cho s n tt cu king rung.

[1]

Asparagus t chi ln v
kinh t cao b nhim sc th 2n = c trng khp th gin
gc ch yu t 
[17]
. 21

Th giu ging,

 trp   ng 600 - 900
i mt bi
o
C - 20
o
 ng tin b k
thu sinh ho gic nhng
gi n tu nhi
nhi 
o
C - 30

vn thu hoc sn pht nh (2 -
 m, hiu qu kinh t r 22

p kht Nam hin nay n ging F2 lai to t 
c sn xut ti Hoa K, ph bin thu sau: Martha Washington,
Mary Washington, California 301, California 500, UC-72, UC-157, UC-309, Jersey King,
Jersey Titan, Greenwich, Apollo, Atlas, Grande, Dulce Verde, Sweet Purple, Purple
Passion
[5,6]

Ging  C ng UC--c lai
to t n xut ti Hoa Kng th nghi c ta cho
thy ging UC-n tt, chng
 t 10-15 t t 15-20 t 3
  -30 t  
i trng.
  i r ging (crown) ly t b r 
 m i, t, ch y t
sn t n thi gian tr  thu hoc
sn phm m - t t sn xut.
2.2.2 Sn xung
Mt trn chun b khong 400 - 500 m
2
 t cao
c tt, lc nng t ng 
cn phi chun b sng trong mt nhuy lc n
phc trnh h

Bng, khong 5 - 6 gi y ht git sch ri
c nh (nhi khong 50
o
C  60
o
n 11 - 12 gi
t ht sch ri thay 1 lnh; tin khong 17-18
gi chi- 24 gi khuya li vt ht sch ri ln
c nh (nhit  khong 40
o
C - 50
o
C).
n 5 - 6 gi t ha tht st bo qun
ging, ht giu t   s tr v  c ri
 i sch buc l khong 18 - 24 gi ht ging s ny nanh
mm r con. Nu gp thi tit lnh, thi gian ny mm ca ht gi - 4
tun hoa (gng h h tr  m bng bin
-60 watt).
n nhng ht giy mm, x i ch phm GA3 ho 
n r, rc l
- ng 4,5 -  
ho- ng khong 4 - 
tr  ch  
p ht ging ny m  phn r n nh tay
t h- a mt b ri lp nh bng mt lp tro
trng 0,5cm cho khut hi nilon mt 24

.
Nh c tuyn chn rt k ngay t n xut git sn xut
bao gi ng 80-90 % s -20 % s ng
 c kho m   ch
nhing 20-i trth tn d
c y hng lai t tr
i tr thu ho
i F2 rt thp)
[1]
.
Sn phm c
ng c i
mng ng), khi
mc cao khi mt, nh ting trc tip chiu x n
  
 cao ti 1,5-
[32], [34]
.
ng mt l cho thu hoch t  sng
th n 20- r ng v t phc hi l
n lch cao nhng tp trung  
  6 - th 7, tu t trng
  tri.

Trích đoạn Ứng dụng của nano bạc Thí nghiệm 7: Khảo sát ảnh hưởng của nano bạc lên sự nhiễm Ảnh hưởng của nano bạc lên sự sinh trưởng của chồi in vitro

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