BỘ GIÁO DỤC VÀ ĐÀO TẠO
TRƢỜNG ĐẠI HỌC NÔNG LÂM TP.HCM
BỘ MÔN CÔNG NGHỆ SINH HỌC
***000***

PHẠM TRẦN XUÂN HIỀN
KHẢO SÁT ĐẬM ĐỘ VÀ KHẢ NĂNG SINH ĐỘC TỐ
CỦA VI KHUẨN Staphylococcus aureus
TRÊN MÔI TRƢỜNG NUÔI CẤY Luận văn kỹ sƣ
Chuyên Ngành: Công nghệ sinh học

T

9/2006

MINISTRY OF EDUCATION AND TRAINING
NONG LAM UNIVERSITY, HCMC
DEPARTMENT OF BIOTECHNOLOGY
RELATIONSHIPS BETWEEN THE BACTERIA DENSITY
AND ABILITY PRODUCING STAPHYLOCOCCAL
ENTEROTOXIN (SEs) OF Staphylococcus aureus
IN TSGM AND BHI BROTH
Engineer Thesis
Major: Biotechnology

Research adviser Researcher
 
Term: 2002 - 2006
 TÓM TẮT KHÓA LUẬN

           
 
Staphylococcus aureus 

ThS. 
Staphylococcus aureus 











S. aureus  
(
S. aureus 
 
                
 


S. aureus.
S. aureus 


ABSTRACT

Staphylococcus aureus is a Gram-possitive coccus having ability to produce
enterotoxin. It is reponsible for one of the most common types of food poisoning. In
testing food and finding the reason causing food poisoning nowadays, we have mainly
testsed the present of S. aureus, not enterotoxin which is the primary caussative agent
of Staphylococcal food poisoning. Therefore, in oder to get information of producing
enterotoxin, we carry on examining S. aureus growth and its ability to produce
enterotoxin in culture medium that contributes practically in testing food, especially in
Staphylococcal food poisoning outbreaks. S. aureus strains are cultured in TSGM and
BHI medium; then we examine their growth and test SE by ELISA method with
TECRA kit after 16, 24, 48 and 72 hours.
The results we find:
Among 36 strains surveyed, there are ten ones having ability to produce
SE (27,8%). In these ten strains, the ones from clinical samples have the highest rate
(50%).

1.1.  ............................................................................................... 1
1.2.  ................................................................................................. 1
1.3.  ................................................................................................... 2
2. TỔNG QUAN TÀI LIỆU
2.1. GiStaphylococcus .................................................................. 3
2.1.1.  ......................................................................................... 3
2.1.2.  ......................................................................................... 3
2.1.3.  ......................................................................................... 4
2.2. Staphylococcus aureus ...................................................... 4
2.2.1.  .......................................................... 4
2.2.2. .............................................. 6
2.2.3.  ......................................................... 7
2.2.4. S. aureus .......................................................... 8
2.3. Staphylococcus aureus ..................................... 8
2.3.1. Nh .................................................... 8
2.3.2. S. aureus ................................................... 9
2.4. Staphylococcus aureus...................................... 11
2.4.1.  ........................................................... 11
2.4.1.1.  ................................................... 11

2.4.1.2.  ................................................ 12
2.4.2.  .................................................................. 13
2.5. Staphylococcus aureus ...................................... 14
2.5.1.  .............................................................................. 14
2.5.2.  ............................................................................. 14
2.5.3.    .............................. 16
2.6. Staphylococcus aureus ............................... 18
2.6.1.  ........................................................................................ 18
2.6.2.  ...................................................................................... 18
2.6.3.  ...................................................................................... 19

4.2.1.  ......................................................................................... 39
4.2.1.1.  ...................................................... 39
4.2.1.2.  ........................................................... 40
4.2.2.  ................................................................... 42
4.3.  .......................................... 44
4.3.1.  ......................... 44
4.3.2.  ................ 47
4.4. SE .................................................................... 47
5. KẾT LUẬN VÀ ĐỀ NGHỊ
5.1.  ................................................................................................... 50
5.2.  .................................................................................................... 50
6. TÀI LIỆU THAM KHẢO ............................................................................... 51
PHỤ LỤC .......................................................................................................... 56

DANH SÁCH CÁC CHỮ VIẾT TẮT

BHI : Brain Heart Infusion
BP : Baird Parker


BẢNG TRANG
S. aureus, S. epidermidis Micrococci ................ 6
S. aureus .............................. 36
S. aureus ..................................................... 37
S. aureus
 ........................................................................ 39
4 S. aureus
 ............................................................................. 41
5.  ................................. 42
S. aureus ch .......................... 44
7. S. aureus  .... 44
8. S. aureus  ........ 45
9S. aureus ................................................. 48



PHẦN 1. MỞ ÐẦU

1.1. Đặt vấn đề
               Tuy

 Theo
  
s. 
Staphylococcus aureus S. aureus 

S. aureus  enterotoxin 

o
C tron
S. aureus 3-
   
 -  





Staphylococcus 
-37
o



               
Iandolo, 2000).
2.1.3. Phân loại
         Staphylococcus    
               
Staphylococcus 
- 
- 
- 
Staphylococcus Staphylococcus aureus 

2.2. Giới thiệu về Staphylococcus aureus
2.2.1. Hình thái, đặc điểm sinh hóa
Staphylococcus aureus   Staphylococcus     
staphylococcus. S. aureus 
-

. Hình 2.1. Hình thái Staphylococcus aureus


 S. aureus 
-
          


S. aureus 
--
5 mm (do k
2
TeO
3


              

 (Mary K. Sandel

S. aureus 

o
  -37
o
C

Bảng 2.1. Những đặc tính của S. aureus, S. epidermidis và Micrococci


S. aureus 
S. aureus 
           
J Harvey
, 2000)S. aureus 
o
C
-

- S. aureus 
S. aureus 

 
S. aureus 


S. aureus 
S. aureus               
Lancette, 2001).
2.2.3. Tính kháng thuốc kháng sinh
S. aureus 

Staphylococcus aureus

Enterococcus faecalis 
S. aureus 
a, S. aureus 






-8 gi-2




G. Normanno , 2004).



                
-48
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


L. McKillip, 2002).
2.3.2. Tình hình ngộ độc do Staphylococcus aureus

 
          u USD. Trong





-
Staphylococcus aureus  


                 



S. aureus 


S. aureus 





S. aureus 

-
--


S. aureus 

-

          

2.4. Phƣơng pháp phát hiện Staphylococcus aureus
2.4.1. Phƣơng pháp truyền thống
2.4.1.1. Phƣơng pháp định tính

o

o 
       
37
o
C trong 24-S. aureus -
-4

(
o 
 TSA (Tryptic soya

o


o 
S. aureus 








Phƣơng pháp MPN (Most Probable Number Method)
S. aureus S.
aureus 




-IS256 PCR, spa t-
         
       S. aureus    -RFLP
aro
ctv, 2004).
2.5. Các yếu tố độc lực của Staphylococcus aureus

Trích đoạn Độc tố ruột enterotoxin của Staphylococcus aureus Hoạt tính gây nôn Tuyển chọn các chủng S aureus Xác định độc tố ruột enterotoxin Khả năng sinh độc tố

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