
TR 

************* 



S 








Metarhizium anisopliae 



 KHO 












Metarhizium anisopliae 




GEN Pr1 -ITS



 TS, ThS. , CN. Lu Ph L 

 





 
, 





 

 



















iv
T


 Metarhizium
anisopliae Pr1 -ITS  

.


 

Metarhizium anisopliae 
. 










Pr1 -ITS

-  Metarhizium anisopliae 










Metarhizium anisopliae var. anisopliae.
- Pr1 10 Metarhizium anisopliae. 




Pr1 10 

. Pr1 7 



1091bp.
-  





94-99% 





Pr1
-  4  2  9 




. 











1.
- 5.8S v2 

 iii
 iv
 v
 viii
 ix
 x
1. 



 1
1.1.  1
1.2.   2
1.2.1.  2
1.2.2. 

 2
 2
2.  3
2.1. 



Metarhizium anisopliae 3
2.2.  6
2.3.  7
2.4. 
2.5. Metarhizium anisopliae
 8
2.5.1.  8

















Metarhizium anisopliae 



Pr1 -ITS 12
2.9.1. 





 12
2.9.2. 






 Metarhizium
anisopliae 18
3.4.2. Pr1 18
3.4.3. 



-ITS 21
3.5. 









Pr1  22
3.6. 





 22




 28
4.6. 





1-5.8S-ITS2. 28
4.7. 









ITS1-5.8S-ITS2 29
4.8.  Pr1 36
4.9. 







RNLA : 
ng : nano gram
nm : nano mol
g : micro gram
m : micro mol
M 
l : micro 
TE : tris EDTA
dNTP : deoxyribonucleotide  5  trphosphate
TAE : tris acetic EDTA
UI : unit international
bp : base pair
Kb : kilo base
ctv 
PCR : Polymerase Chains Reaction
Rpm : Revolutions per minute ix
NG


 3.3  Pr1 




METPR2/METPR5 20
 -ITS .21
Bng 3.5  23
Bng 4.1  Metarhizium anisopliae 














-ITS. 30
4.2 








Metarhizium anisopliae trong




 











-ITS 33
4.4 Metarhizium anisopliae 






Pr1 37
4.5 






Metarhizium anisopliae trong




 









Pr1 40 x


 TRANG

Metarrhizium anisopliae 
nhau 5
2.2 

3.3 











 24
3.4 









 25
DNA tng Metarrhizium anisopliae 26
4.2 






Metarhizium anisopliae 
 gi









-ITS 34
i gen Pr1 36
Metarhizium anisopliae c
d gen Pr1 39
4.9 



Metarhizium anisopliae 
 gi







gen Pr1 41

         RAPD, RFLP, AFLP, Southern blot,
Sequence v.v 

, Bm
s 
Metarhizium anisopliae  Pr1 -
ITS .
1.2.  
1.2.1.












 Metarhizium anisopliae












 Metarhizium anisopliae








.
- Pr1 -



.
-   Pr1  -













Clavicipitaceae

Metarhizium

Metarhizium anisopliae




 


 


4

Metarhizium anisopliae  : M. anisopliae var. major 
  14,0 (18,0) M. anisopliae var. anisopliae
  8,0) m. 
Metarrhizium anisopliae) 

 Metarhizium anisopliae          

 

Metarhizium anisopliae 
           
  Metarhizium anisopliae        

2

               Aeromonas 
in vitro).
- 
o

o

-  30
o

o
C trong

- 
o
 8,5.
- Metarhizium anisopliae    




7



n.





 

 


    
 




2.3. .

  Beauveria bassiana,    : Metarhizium
anisopliae .Trong 
.
2.4. 
 Metarhizium anisopliae  Beauveria       


     
Long.


             Beauveria bassiana,
Metarhizium anisopliae  Normurea rileyi
              
Hemiptera.
2.5.2. 

     
ang, 1997).
           

-
        Pandora neoaphidis,
connidiobolus thromboides, C.coronatus, Entomophthora planchoniana  neozygites
fresenii) Beauveria basssiana, Verticillium lecanii).

          Metarrhizium anisopliae, Beauveria
bassiana  Verticillium lecanii 
 
                
Metarhizium anisopliae Beauveria bassiana  
Verticillium lecanii 

10


Metarhizium anisopliae 



1996).



 
      
                

             

     
rRNA .






 -LSU-
     
2001).
12




     








 Metarhizium
anisopliae 



Pr1 -ITS
2.9.1. 






Savita Bagga, Gang Hu, Steven E. Screen, Raymond J. St.













Nested-











 Pr1A Pr1B 










 



  .







. 









 t Pr1 








n .
Sorayac C . M 

 (1997) 








 Pr1  





 . 




40 Metarhizium 



15 
4 .


 (2002) 





 Metarhizium anisopliae 
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
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
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
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


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
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
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
.
Malena P. Panton, Annoula Mavridou, Milton A.Typas (2003) 
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
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
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

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
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

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
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
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
RFLP-
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
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


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 (rDNA) 
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
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
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
Metarhizium  M.anisopliae var. anisopliae, M.anisopliae var. majus,
M.album M.flavoviride .
14

Marilena Aquino de Muro, Samina Mehta, David Moore (2003) 
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
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


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
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

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. 
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
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
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
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
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
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 , 25 d 20
.

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 (2000) 
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
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
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

 . 
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
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
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
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 10 

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.
Curran  (1994) 
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
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
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
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
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 
Metarhizium 
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


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



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
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
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
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 Metarhizium
anisopliae 
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
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 Pr1  -ITS  . 
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
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

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
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
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

 
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Metarhizium anisopliae 