46
Điều chỉnh pH = 7,4 bằng NaOH 1N hoặc NaOH 5N.
(f) Môi trƣờng khi nuôi
FCS 200 μl
Pyruvic acid 200 μl
hMG 200 μl
TCM 199 1400 μl
2. Trên chó
(a) Thành phần môi trƣờng
TCM199 môi trường căn bản
HEPES 25mM/l
ECS 10%
Gentamycin 50 μg/ml
NaHCO
3
2,2 mg/ml
Pyruvic acid 22μg/ml
Estradiol 1 μg/ml
FSH 0,5 μg/ml
hCG 0,03 UI/ml
(b) Pha môi trƣờng căn bản TCM199
Nước cất 100ml
TCM199 980 mg
NaHCO
3
220 mg
Gentamycin 5 mg
Hepes 5,97g
Trữ ở 4
o

NaCl 800 mg
KCl 20 mg
Na
2
HPO
4
.12H
2
O 290 mg
KH
2
PO
4
20 mg
Polyvinyl alcohol (PVA) 100 mg
PVA được pha riêng với nước cất, gia nhiệt khoảng 70
o
C đến khi PVA tan hết trong
nước. Sau đó được pha chung với môi trường PBS – PVA.
Table of Least Squares Means for HEO.LOAIA 95%
Confidence
Level Count Average Stnd. Error for mean GRAND MEAN 177 .4825215 .0478110 .3881326.5769104
A:HEO.PPHAP
1 84 .4960188 .0556274 .3861985.6058390
2 93 .4690242 .0536471 .3631135.5749349
B:HEO.KTHUOC
1 90 .4164220 .0370971 .3431846.4896594
2 80 .6235000 .0392682 .5459763.7010237
3 7 .4076425 .1328045 .1454584.6698265
Table of Least Squares Means for HEO.LOAIB 95%
Confidence
Level Count Average Stnd. Error for mean GRAND MEAN 177 .3639194 .0425073 .2800011.4478377
A:HEO.PPHAP
1 84 .3535265 .0494567 .2558887.4511643
2 93 .3743123 .0476960 .2801503.4684743
B:HEO.KTHUOC
1 90 .4377429 .0329819 .3726297.5028560
2 80 .2755000 .0349121 .2065761.3444239
3 7 .3785153 .1180724 .1454155.6116151 Table of Least Squares Means for HEO.loaiC 95%
Confidence
Level Count Average Stnd. Error for mean GRAND MEAN 177 .1619216 .0355520 .0917345.2321087
A:HEO.PPHAP
1 84 .1711807 .0413643 .0895189.2528425
2 93 .1526625 .0398918 .0739078.2314172
B:HEO.KTHUOC
1 90 .1691564 .0275852 .1146974.2236153
2 80 .1010000 .0291996 .0433538.1586462
3 7 .2156084 .0987528 .0206497.4105672

TOTAL (CORRECTED) 3.5487134 53 0 missing values have been excluded.
All F-ratios are based on the residual mean square error.

Table of Least Squares Means for CHO5.A 95%
Confidence
Level Count Average Stnd. Error for mean GRAND MEAN 54 .7903141 .0377690 .7144723.8661558
A:CHO5.buongtrung
1 26 .7855320 .0557147 .6736546.8974095
2 28 .7950961 .0498658 .6949635.8952287


RESIDUAL 3.5399402 51 .0694106 TOTAL (CORRECTED) 3.5487134 53 0 missing values have been excluded.
All F-ratios are based on the residual mean square error.
Table of Least Squares Means for CHO5.B 95%
Confidence
Level Count Average Stnd. Error for mean GRAND MEAN 54 .2096859 .0377690 .1338442.2855277
A:CHO5.buongtrung
1 26 .2144680 .0557147 .1025905.3263454
2 28 .2049039 .0498658 .1047713.3050365