y
Bacillus
probiotic :
: 62 73 01 01
- 2014
Trong
.
, Bacillus
,
Bacillus sinh carotenoid .
ny Bacillus spp.
probiotic , :
1. Bacillus sinh carotenoid,
2. .
3.
4.
Bacillus
5.
6.
2
139 6 trang,
- - 1
72 .
3 118 1
7
1.1.
1.2.
1.3.
1.4.
1.5.
1.6. BacillusBacillus,
1.7.
1.8.
Bacillus sinh carotenoid.
Bacillus sinh carotenoid.
4
T
theo M7 -
tetracyclin, quinolon, phenicol, ansamycin.
- 45
o
- 11.
(Knauer -
acetonitril:methanol:dicloromethan (7:2:1, tt/tt), RP18 (250 mm, 4,6
mm, 5 m); UV-Vis,
450nm
o
l. X
1 erlen.
HPLC (
cho TSB:
,
sung K
+
, Mg
50
: DD1.1; AT14.
: NaCl 0,85%, HU58 (B.subtilis). 10
7
/g TTC.
- .
-
-
DD1.1; AT14.
: NaCl 0,85%, HU58 (B.subtilis), -caroten). 10
6
/g TTC.
.
2.4.8.1.
.
: : DD1.1, AT14 (10
6
5x10
8
/ml).
7
- 50%.
-
:
AT14, AT22, CG17.0,
3.3.
o
C, pH 7-9.
-.
(log
10
()
R
2
()
AT14
0,346
0,870
0,956
13-34
AT22 Độ hấp thu (mAU)
0
5
40
20
10
10.1
5.1
DD1.1
0
5
40
20
10
8.1
3.9
CG17.0
10
40
20
0
3.0
3.9
AT22
0
8
4
0
PY79
0
25
100
75
50
9.9
5.1
8.1
HC28
Bacillus
3.4.2.
(DD1.1, AT14, HU36 (AT22, CG17.0,
HC28).
g/g SKK)
() *
AT14
745
22 24
MTTT, b
•
•
, K
+
, Mg
2+
, Mn
2+
, Ca
2+
,
Fe
2+
AT14, DD1.1.
pO
2
(%)
AT14
5 ml NaCl 0,85%
7 L K15
agar 105 g
72,94 ml KCl 10%
10,42 ml MgSO
4
12%
10,5 ml Ca(NO
3
)
2
1M
6,86 ml MnCl
2
10 mM
5,94 ml FeSO
4
1 mM
o
C,
o
C, 200 , 12
, 350 ml TSB
37
o
C, 200 , 12
o
C, trong
48
5 ml NaCl 0,85%
7
105 g agar
76,86 ml KCl 10%,
10,22 ml MgSO
4
12%,
10,36 ml Ca(NO
3
)
2
1M,
8,26 ml MnCl
2
10 mM,
4,55 ml FeSO
4
1 mM
12
8 L
77,52 ml KCl 10%
12 ml MgSO
4
12%
5,76 ml Ca(NO
3
)
2
1M
4 ml MnCl
2
10 mM
8,56 ml FeSO
4
1 mM
o
C,
AT14
37
AT14
men
men
57,76 ml KCl 10%
10,64 ml MgSO
4
12%
9,52 ml Ca(NO
3
)
2
1M
8,72 ml MnCl
2
DD1.1
men
men
4DD1.1
13
21,32
17,65 (82,8)
16,11 (75,6)
55,95
1,1
AT14
2
22,11
16,87 (76,3)
16,49 (74,6)
54,40
1,2
3
21,17
17,01 (80,3)
16,74 (79,1)
54,68
1,1
TB
21,53
17,18 (79,8)
16,45 (76,4)
55,02
1,13
1
96,03
84,07 (87,5)
men
x 10
12
CFU
x 10
12
CFU (%)
x 10
12
CFU (%)
(g)
Carotenoid
(g/10
9
BT)
1
39,14
31,36 (80,1)
30,29 (77,4)
86,86
1,51
AT14
2
39,32
161,07
0,43
3
73,01
64,56 (88,4)
60,38 (82,7)
159,80
0,41
TB
72,33
64,85 (89,7)
61,37 (84,8)
160,43
0,42
.
:
g 3.6. MTTT
ng
(cho
DD1.1)
(cho AT14)
Thch
DSM
7.300
LD
50
>10
11
b />5x10
12
b
. Sau , c
,
3.10. thu,
, DD1.1 carotenoid trong gan
cao
VK .
15
.
B. infantis
B.marisflavi
6
#10
8
72
16
Bacillus
-
-
,-
-
-caroten,
38
chi Bacillus
B. infantis, B.
ferrariarum, B. catenulatus, B. vietnamensis, B. boroniphilus, B.
baekryungensis, B. alcalophilus, B. selenatarsenatis B. mangrovensis
Bacillus
18
AT14:
(
DD1.1:
4.3.
Bacillus sinh
carotenoid
(10
9
.
-3
130
10
9
1,52
/>5x10
12
.
11
12
cao so
.
20 4.5.
carotenoid trong gan
cao
VK B. subtilis
HU58.
Bacillus
• C
•
n
DD1.1.
Bacillus sinh
i