B Y T
I HI
NGUYN TH THU TRANG

U CH MAGIE
LACTAT B
TNG HP N TT NGHI
I - 2014
B Y T
I HI
NGUYN TH THU TRANG

U CH MAGIE
LACTAT B
TNG HP N TT NGHI


MC LC

LI C
MC LC
DANH M VIT TT
DANH MNG
DANH M  TH
T V
NG QUAN
1.1. Magie lactat
1.1.1. 
1.1.2. 
1.1.3. Mt s sn ph 
1.2. n xut magie lactat
1.2.1. ng h
1.2.2. 
1.3. Vi khun sinh axit lactic
1.3.1. 
1.3.2. Chi Lactobacillus
1.3.3. Lactobacillus acidophilus
1.3.4. 
1.4. 
U
t liu, thit b
t li
2.1.2. Thit b s d


u su 
2.3.8
C NGHIM, KT QU N.
3.1.  o magie lactat 
3.1.1. La ch
3

3.1.2. La chn ph
3.1.3. n phm m
3.2. Kht s  
3.2.1. Khng c
3.2.2. Khng ca n 
KT LU XUT
U THAM KHO
PH LC
16
16
17
17
17
17
18
18
18
19
21
21
22
22
22

Lactobacillus bulgaricus
L. casei
Lactobacillus casei
L. delbrueckii
Lactobacillus delbrueckii
L. fermentum
Lactobacillus fermentum
L. leichmenii
Lactobacillus leichmenii
L. plantarum
Lactobacillus plantarum
S. lactis
Sacharomyces lactis
M
Kh

 lch chun
R
f
H s di chuyn
x
 

V
WHO
World Health Organization DANH MBNG


4
14
15
15
16
23
24

25

27

29

35
37

38

39

DANH M  TH Trang
L. acidophilus n vi quang hc
L. acidophilus n t


35
37
1

T V
n pha vi sinh vc ng
dng ph bin trong mc ci sng. n phm c
ngh t, axit i lactat ng sn phi
t rt sng dng r Lactobacillus acidophilus c quan
u nhit trong nhng chng vi khu
y  p [4]nh canxi lactat vc nhc
te lactat t mui lactat ng dc
phi l ln [1], [8].
t cn thit cho s n th l ca
   magie  c t     p
Magie duy nh  nu
u ch t v quan trn thit.
agie lactat c s dng rt ph bing cha bnh,
magie lactat n sn phn xut canxi
lactat, axit   ng sn ph     s dng trong
p thc
phm, m ph [16], [22].
 git sn xut magie lactat 
mn.  Vi 
n, nhu v kh ng hp
u. Vi mong mu

dng dihydrat (C
6
H
10
MgO
6
.2H
2
O) 
6
H
10
MgO
6
.3H
2
O).
 t: Magie lactat tn ti dng k
c lnh, tan tt trt tan trong
c ln 96
0
[23].

1.1.2. ng
Magie lactat c s dng trong nhic:
 Trong Y hc:
- t t n thi  h tr
cha h tim mch, th c
ng thng phi hp vi Vitamin B6.
- c s du tr thiu mc

p dt, tng hp vecni, cht do, nhng nhit.
4

- Magie lactat c s d  tn
n t, thit b t  [22]

1.1.3. Mt s sn ph ng
t smagie lactat
STT



1
Maglek B6
Magie lactat,
Pyridoxin

Lek-AM, Poland Lek-AM,
Ba Lan
2
Magne B6
Sanofi-Aventis, Lithuania,
Sanofi-Aventis, Malta;
Sanofi-Aventis, Ba Lan
3
Magne B6 Stada
Stada-VN JV, Vietnam
Stada-VN JV, Vit Nam
4
Magne - B6

12
Magnesium
NYCOMED, Romania
13
Oromag
Theramex, Tunisia
14
Magnegon
Takeda Pharma, Th
15
Wamag
Magie lactat, hn
hp mui canxi
Hasco, Ba Lan

16
Magnesium
Glycocolle
Lafarge
Magie lactat,
glycin
SERP, Monaco 1.2. n xut magie lactat
1.2.1. ng hc
5

Bng h
cu tng h c magie lactat t      nhau 

ng hng
m cc hi, d y, nhi sn xut thp,
ng sn xut thp, tn dn phm tha cc
phm, vn phm r c sn phm tinh khi
[21], [24].
- m:
+ To sn phm t  axit L (+) lactic [21].
+ Tn dt th
nghip sn xuo, ch bin sa, g [24].

1.3. Vi khun sinh axit lactic
1.3.1. n sinh axit lactic
Vi khun sinh lactic gc khun hay cu khun Gr (+), t l GC
) thp, chc axit 
hoc vi hia v
i chy
trong thc vn phm ca axit m chung ln
nht ca vi khu axit l
sn phi cht cu [3].
Vi khun sinh lactic ch yu thuc 4 chi: Lactobacillus, Pediococcus,
Streptococcus, Leuconostoc; t s m thuc chi Rhizopus
[1].
c ca vi khun sinh lactic rnc
tc mi.
7

a vi khun Gram
, hn y
hoc vi hi dnng t t ra axit lactic.
n ti tt  pH thp (4,5  6,8), nhi khong 10 

.1. L. acidophilus 
hin vi quang hc

 1.2. L. acidophilus  
hin t
L. acidophilus c khun Gram (+), dc
c to chui ngng, phn ng catalase
h; i khut, nhi t  45
0
C, chu
i thp (5  6); hp hi  y
 n ph [3],
[8].
L. acidophilus         i trong h
thng li vi khu vi
khui. L. acidophilus p t dch rut tr ch ru
Hin nay, L. acidophilus c s dng nhi ph
 u tr ng hp ri lo
tr mm ho [9].
L. acidophilus thun phm cui
axit c ng dng rrong thc tio qun thc
phm, dung dch v sinh ph n (D 

9

racemic. Dng axit racemic  t qu t 
tng h hp cht lactonitril hoc nh hong ca enzym DL
 lactatdehydrogenase do mt s  sinh v ng hp
axit lactic t [26].
10

axit t khi b 
d     chuy  ng axit    ng ca
enzym racemase t mn lactic.
+ t ca axit lactic:
Dng dung dch: axit t lng trong su
 
Dng tinh th: d tan  n to cht lng.
 pha lng: axit lactic d chuyn sang dng dimer mch thng lactoyl
ch th
  t ester v       ng dimer mch
 c thc hi
 140
0
t th b  quay cc.

1.4. u agie lactat
Peter Johannes Marie Baets ng s (2013)   
sn xut mui lactat b nguu thc vt,
m 25% cacbohydratt thc v
Mg(OH)
2
agie lactat [25].
Jan Van Krieken vs dng 
Mg(OH)

 gi canxi lactat 
to ra canxi sult thi rn (th

agie lactat
 

2

 
canxi
lactate
 

      
 

axit
la clorua.
12

Sinh


Aharon Meir Eyal, Rod Fisher (1998) s

    Lactobacillus delbrueckii  Lactobacillus
acidophilus. libohydrat
 
              5,
t -   - 6,5

vin ti Vi 
n xut


14

U

t liu, thit b
2.1.1. vt liu
 Chng ging: Lactobacillus acidophilus ATCC 4356 d  
(ngun gc: Vin Kim nghim).

(NH
4
)
2
SO
4

Trung Quc
Na
2
S
2
O
3

Trung Quc
KH
2
PO
4
Trung Quc
KMnO
4
Trung Quc
MnSO
4
.H
2
O
Trung Quc

Trung Quc
NH
3
Vit Nam
Axit oxalic
Trung Quc
Br
2
Trung Quc
CuSO
4
.5H
2
O
Trung Quc
CuSO
4
.5H
2
O
Trung Quc
NaK(C
4
H
4
O
6
)
Trung Quc
HCl

4,0g
Sa bt
1,0g
Cao nm men
0,4g
(NH
4
)
2
SO
4

0,2g
KH
2
PO
4

0,25g
MnSO
4
.H
2
O
0,001g
FeSO
4
.7H
2
O

2
PO
4

0,25g
MnSO
4
.H
2
O
0,001g
FeSO
4
.7H
2
O
0,001g
MgSO
4
.7H
2
O
0,03g

100ml
u chnh pH = 6,3  6,5  ch s dng
ng dn c

2
SO
4
98% pha vi

2
i.
16

 Dung dch Na
2
S
2
O
3
     
2
S
2
O
3
 
Na
2
CO
3
c c
2

2

nh mc, pipet, ng nghing c
pha ch

2.2. Nu
2.2.1.  ng o magie lactat
 La chn agie cacbonat
- B
- 
17

- K
 La chn thu sn phm magie lactat
- Lng phu lc Buchner.
- ng.
- nh.
  sn phm magie lactat
- 
2+
.
- 
- Sp mng.
2.2.2. Kht s  
 Khng ca pH: 6  8.
 Khng ca n glucose: 7  12%.

u
ging:
  ng  .2)  c tit   0,6 atm
  ngui xung 40  45
0