i

--------------

3333333333333333333

CH

QUY TRÌNH S
XU
N NG T N
LINH CHI

VIÊN NANG TH
PH
D
SÓNG SIÊU ÂM

: Chính quy
Chuyên ngành/Ngành : CNSH-CNTP
: CNTP
: 2011-2015


i

L

Thái Nguyên, ngày 29 tháng 5
Sinh viên


..............................................................................................................2
...........................................................................4
........................................................................4
............................................................................4

2.1.2. Phân lo

.....................................................................................4
................................................................5
.6
.............................................................6
......................................................6
a- Glucal và Triterpenoid..........................................................9

2.3.1. Beta-glucal ......................................................................................................10
2.3.2. Triterpenoid.....................................................................................................11
........................13
...........................15
2.4.1.

trích ly

............................15
- Glucal và Triterpenoid trong

17
18

.................................20
.........................................................................................20


Linh Chi.....................................................................................................................39
Beta...................................................................................................................................41
-

.41
-glucal

.........................................................................................................43
.............................................................................................................45
..............................................................................................46
-gluca
Linh chi .....................................................................................................................47
................................................49
4.3.1.

...................................................................................................49


v

............50
4.3.2. X

................51
..................................................................53
................................................................................................................53
..............................................................................................................53



ng c a nhi

t i kh

-glucal trong n m Linh

Chi..........................................................................................................44
Chi .............................................................................................................................43
B ng 4.10.

ng c

sóng siêu âm t i kh

-glucal

trong n m Linh Chi..............................................................................47
..............................................................................................46


vii

-gluca
.............................................................................................................47
Linh chi .....................................................................................................................48
...............................................50
...............................50
16

viên nang Linh chi ..................51

ta........................................................................................44
Hình 4.9
-glucal
...................................................................................................45
Hình 4.10
..............................................................................................46
Hình 4.11
Triterpenoid
.........................................................................................................49
.............................................................................................................48


ix

Ýn

Cm

: Centimet

CT

:

Da

: Dalton

g



inh


2

1.2. M

u

1.2.1. M

1.2.2. Yêu c u

Triterpenoid
-

trích ly

-

trích ly
trích ly

-

trích ly

-



2.1.2. Phân lo i n m Linh chi

Ganoderma lucidum

regnum

(Fungi)
(Basidiomycota)

ordo): Polyporales
Ganodermataceae)
Chi (genus): Ganoderma
Loài (pecies): Ganoderma. Lucidum

-

Ganoderma applanatum (Pers) Past,


5
-

Ganoderma lucidum (Leyss ex fr) kart

m th c v t c a n m Linh chi
trên các

-



-2,1%;


7
-

Nhóm steroide
-

sterol [21].
-

Ganodermenonol: : 26 - hydroxy - 5 alpha - lanosta - 7,9 (11).24 - trien -one
Ganodermadiol: 5 alpha-lanosta-7,9 (11).24-trien-3 beta.26-diol
Ganodermatriol: 5 alpha-lanosta-7,9 (11).24-trien-3 beta.26,27-triol.
-

Steryl este 1: Ergosta-7.22-dien-3beta-yl-linoleate
Steryl este2: 5alpha. 8alpha- epidioxyergosta- .22-dien-3 beta-yl-lino leate.

- Nhóm polysacharide

gây ung

-Dacid

-

-proteine


C

Ganodosteron
Steroid

Lanosporeric A
Lonosterol
Compounds I, II, III, IV, V
Ganoderans A, B, C

Polysacharide

Beta-D- glucan
BN-3B:1, 2, 3, 4
D-6
Ganoderic R, S
Ganoderic B, D, F, H, K, Y
Ganoderic s
Ganodermadiol
Ganodermic Mf

Triterpenoid

Ganodermic T.O
Lucidone A
Lucidenol
Ganosporelacton A
Ganosporelacton B


Beta-Glucal
-

ành có

-1,3-glucal
-1,3-glucal
ra, theo các n

inh chi
-glucal.
ng c a Beta-glucal

Beta-Glucal

-glucal
-


11

-

-

ch.
2.3.2. Triterpenoid

-


có
,

ho

u.

Hyun, Choi, và Kim (1990)

-81% so

-

.

[25].
2.3.1.2.

ng d

n m Linh chi

u tr

i v i h th ng mi n d ch các ho t ch t sinh h c trong


14
n



trong n m Linh chi

2.4.

ng d ng sóng siêu âm trong trích ly các ho t ch t sinh h c

2.4.1.

khoa h c trích ly các ho t ch t trong n m Linh Chi
-Glucal trong

polysacharidee Linh Chi. Polysacharide
guaran gum,

trích ly polysacharide
0

là

1/12.
75% [25].

và c

s

50W trong
là 1/