1 BỘ GIÁO DỤC VÀ ĐÀO TẠO
TRƢỜNG ĐẠI HỌC NÔNG LÂM TP. HỒ CHÍ MINH
BỘ MÔN CÔNG NGHỆ SINH HỌC
***000*** NGUYÊ
̃
N BĂ
̀
NG PHI
THIÊ
́
T KÊ
́
VA
̀
NGHIÊN CƢ
́
U HÊ
̣
THÔ
BỘ GIÁO DỤC VÀ ĐÀO TẠO
TRƢỜNG ĐẠI HỌC NÔNG LÂM TP. HỒ CHÍ MINH
BỘ MÔN CÔNG NGHỆ SINH HỌC
***000***
THIÊ
́
T KÊ
́
VA
̀
NGHIÊN CƢ
́
U HÊ
̣
THÔ
́
NG BIOREACTOR
CHO CÂY DƢ
́



-2006-

3 MINISTRY OF EDUCATION AND TRAINING
NONG LAM UNIVERSITY, HCMC
DEPARTMENT OF BIOTECHNOLOGY
************ DESIGN AND RESEARCH BIOREACTOR SYSTEM FOR
CAYENNE PINEAPPLE
Graduation thesis
Major: Biotechnology Professor Student
TRAN THI DUNG NGUYÊ
̃
N BĂ
̀OR

NGÀNH: CÔNG NGHỆ SINH HỌC
NIÊN KHÓA: 2002 – 2006
SINH VIÊN THỰC HIỆN: NGUYỄN BẰNG PHI

Thành phố Hồ Chí Minh
2006
5


2006 6 

Để hoàn tất cuốn luận văn này tôi đã nhận được sự hướng dẫn, giảng dạy, góp
ý, giúp đỡ của các thầy cô, bạn bè và gia đình. Tôi xin được bày tỏ lòng biết ơn sâu
sắc đến:
-
-- 

- - 


- 


 

         -  
         in vitro.     

- 

  T         


: 
 
  : T           
Temporary immersion bioreactor)


+

+ 

              

8 +Bioreacto
.
+B
  


.................. 38
 ................. 40
 ................................. 44
 ...................... 44
Phần 3 : Vật liệu và phƣơng pháp nghiên cứu
3.1.  .......................................................................................... 45
 ............................................................................................ 45
 ................................................................................................................. 45
 ..................................................................................... 45
3.4.1
 ..................................................................................................... 45
10  ......................................................................................... 48
3. ........................................................................................................ 50
Phần 4 : Kết quả và thảo luận
4.1 
(TIB) ........................................................................................................................... 51
 ........................................... 51
4.1.2  ................................ 51
 ............................................................................................... 53
 
 . .......................................................................................... 53
4.2.2  : 
 ............................................................................... 55
Phần 5 : Kết luận và đề nghị
................................................................................................................ 60
 ............................................................................................................... 60
 ...................................................................................................... 61

Hình 2.1- Cấu trúc bioreactor ............................................................................ 26
Hình 3.1-  .... 42
Hình 3.2-.......................... 43
Hình 4.1- .................................................................................. 47
Hình 4.2-  ........................................... 47
Hình 4.3- ....................................................... 48
Hình 4.4-
 .............................................................................................. 50
Hình 4.5-   ........................... 53
Hình 4.6- ......................................................................... 55 13 DANH MỤC CÁC CHỮ VIẾT TẮT

BAP : 6-benzylaminopurine
GA
3
: Gibberellic acid
IAA : Indole-3-acetic acid
IBA : Indole-3-butyric acid
NAA : -Naphthaleneacetic acid
TDZ : Thidiazuron
MS 
TIB : Temporary immersion bioreactor 14

  Q

, 
 
15 
.
 n

           

1.2 Mục đích - Yêu cầu
Mục đích
          
           
.
 



Yêu cầu


bioreactor (TIB)
1.3 Giới hạn đề tài :
- 
    



Hạt




Thân



Lá
17  
 


 
Rễ



 
Sinh thái cây dứa
                
 32
0
 40
0


 Cayenne

Nhóm Cayenne


 

 

Cayenne 


Nhóm Queen

 



 

19  


Nhóm Spanish

            




Dứa Kiên Giang và dứa Bến Lức (từ địa phƣơng là “khóm”)






Nhóm dứa Cayenne

 

 




 



      

2.4. Tình hình sản xuất và sản lƣợng dứa
Tình hình sản xuất và sản lƣợng dứa trên thế giới
21
           
22  

            


2.5. Giới thiệu về nuôi cấy mô
Nuôi cấy mô tế bào thực vật
in vitro 









Lịch sử phát triển
             





ng tin


- 
- 
- 
- 
- Lai xa.
- 
- 
- 
24 - 
- 
Các phƣơng pháp nuôi cấy in vitro
Nuôi cấy đỉnh sinh trƣởng

0.58 - 



Nuôi cấy mô sẹo

 
                




Phƣơng pháp nuôi cấy tế bào đơn



 Một số chất điều hoà sinh trƣởng thƣờng dùng:
Auxin





 



Trích đoạn Tiến hành thí nghiệm Kết quả thí nghiệm Thí nghiệm 2: Ảnh hưởng của thời gian ngâm chìm định kỳ đến

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