DAI HOC QUOC
GIA
HA
NQl
TRlTOfNG
DAI
HQC
KHOA HOC
TU^
NHIEN
DANG
THI
NGAN HA
NGHIEN
ClTU
TINH
ANTOAN
VA KHA NANG GAY DAP
LTNCi
MIEN
DICH
TREN
KHI
Macaca mulatta
CUA
3
CHUNd
VIRUT
ROTA
HE
GIONG

LOI CAM
DOAN
CAC KI HIEU VA
CHC
VIET
TAT
MUCLUC
DANH MUC CAC BANG
DANH MUC CAC HINH
MCJDAU
1
CHUONG
I: TONG
QUAN
3
1.1 Virut Rota 3
1.1.1
Ljch siJ
phat hien virut Rota 3
1.1.2.
Phan
loai
virut Rota 3
1.1.3. Hinh thai va cau
true
4
1.1.4.
Cac thanh phan hoa hoc cua virut Rota va chuc nang 6
1.1.5.
Phan nhom va typ huyet thanh 10

Duong lay truyen 21
1.2.5.
Milndich
21
1.3.
Tinh hinh nhiem virut Rota tren
Th^
gioi
22
1.4. Tinh hinh nhiem virut Rota tai Viet Nam 23
1.5.
Tinh hinh nghien cuu va phat trien vacxin Rota tren
Th^
gioi
va trong
nuoc
26
1.5.1. Tinh hinh nghien cuu phat trien vacxin tren
Th^
gioi 26
1.5.2.
Tinh hinh nghien cuu va phat
triln
vacxin tai Viet Nam 32
1.6.
Tieu chuin
ciia T6
chuc
Y
t^

1.7.1.
H6
so tom
tk
cac
buoc
cay
truyen chiing giong
goc
G1P[8]
de tao
chiing
ung
vien
cho san
xuat vacxin
42
1.7.2.
H6
so tom tat cac
buoc
cay
truyen chiing giong
goc
G1P[4]
de tao
chiing
ung
vien
cho san

tugng
nghien
cuu 45
2.1.1.
Chiing giong
goc
(Master seed)
45
2.1.2. Chiing
san
xuat vacxin (Working seed)
46
2.2.
Nguyen
vat
lieu
va
phuong phap kiem
tra he
thong chiing
46
2.2.1.
Phuang phap
xet
nghiem
vi
khuan
va nam 46
2.2.2. Phuorng phap
thu

nhan ngoai
lai
tren
te bao
than
khi
tien phat
52
2.2.7.
Thu
nghiem
tac
nhan ngoai
lai
tren
te bao
Vero
53
2.2.8.
Thu
nghiem
tac
nhan ngoai
lai
tren
te bao
Hep2C
53
2.2.9.
Xac

cua
virut
Rota
71
2.2.14. Phuang phap thong
ke
xii
ly so
lieu
77
CHITONG
III: KET QUA VA BAN
LUAN
78
3.1.
Ket qua
kiem dinh tinh
an
toan
he
thong chiing virut Rota
78
3.1.1.
Kilm
tra vi
khuAn
va nam va vi khuan lao 78
3.1.2.
Kiem tra tac nhan ngoai lai trong hon dich chiing virut Rota bang
phuang phap PCR 79