
KHOA SINH - KTNN
======




KHOA SINH - KTNN
======  

GLUCONACETOBACTER



Hà Nội, ngày 12 tháng 4 năm 2014
Sinh viên 





 m,              
         


Hà Nội, ngày 12 tháng 4 năm 2014
Sinh viên 


 1

 17
.  18
3.1. QGluconacetobacter BHN
2
18
 
Gluconacetobacter BHN
2
18
Gluconacetobacter
BHN
2
19
 20
 20
 23
 25
 29
 29
 29
 30
 

A.xylinum
Acetobacter xylinum
ASTM
American society for testing and materials




 4
Gluconacetobacter 7
 21
 23

 25 

Hình 1.1. Gluconacetobacter 11
        Gluconacetobacter BHN
2
 
       Gluconacetobacter BHN
2
trên
MT1 19
Hình 3.2. Màng BC sau 5 ngày lên men 20
 20
 22
 22
 22
 24
 24
 24
Hình 3.10. Nem chua b 24

ch,
 [21], [22], [24].

           
           
 
       
 
 2 phân , Gluconacetobacter BHN
2


“Nghiên cứu một số hướng
ứng dụng màng BC tạo ra từ vi khuẩn Gluconacetobacter”.
Nguyễn Thị Thùy 2

2. Mc tiêu c tài


3. Ni dung
Gluconacetobacter
BHN
2



c và thc tin
4.1. Ý nghĩa khoa học: n vi 

4.2. Ý nghĩa thực tiễn:          

Gluconacetobacter 
Gluconacetobacter nom. corrig, theo 
      Gluconacetobacter  


     Gluconacetobacter     
             
Acetobacter Gluconacetobacteri cùng Yamada (2000)
        Gluconacetobacter    
ng t là
Nguyễn Thị Thùy 4

D - u Q - n ub
            
Gluconacetobacter           


AND.
Theo Bergey (2005) [16], G.intermedius    
Gluconacetobacter 
Acetobacter, Acidomonas, Asaia, Gluconobacter, Gluconacetobacter và
Kozakia


(1)
(2)
(3)
(4)
(5)
(6)

-
w



+

+

-

+

+

+
Sinh  - manitol
+/-
w
+/-
+/-
+
+

-/w
+
-
_
-
-

-
-
Nd
+/-
+
-
+/-
+
-
+
+
-
-
+
+
i ubiqiunone
Q-10
Q-10
Q-10
Q-10
Q-10
Q-10
%mol G + C
52-60
63-66
59-61
55-66
54-63
56-57




CGluconacetobacter 


H
2
SO
4

 c           

.

      Gluconacetobacter hình thành


Gluconacetobacter g

BC. 

 
.

 m sinh lý: C Gluconacetobacter   pH: 4 - 6,
- 35
o

o


3
   


_
4
  
thành dihydroxyaceton

+
5
  
thành acid


3

+
6
    


_
7
    


+

1.2. Nhu cu ng ca chng Gluconacetobacter

           
Nguyễn Thị Thùy 8

             
  
c 0,5 atm 110
o
C 
ng dùng 0,2 - 
.
1.2.2. Nhu cầu nguồn nitơ
(NH
4
)
2
SO
4

    Gluconacetobacter.    
Gluconacetobacter   
4
+
 
NH
4
+

4
2-
, Cl


Gluconacetobacter 

1.3.  hình thành màng BC
1.3.1. Đặc điểm cấu trúc của cellulose và màng BC
1.3.1.1. Đặc điểm cấu trúc của cellulose

5
- 22,68.10
5


6
H
10
O
5
)
n

 

-               
glucose. Cellul
        a các nhóm (- 

c monosaccharide. Cellulose có tính

        
nhau.

12
O
6

Nguyễn Thị Thùy 10



[C
6
H
7
O
2
(OH)
3
]
n
+ 3n HNO
3
-> [C
6
H
7
O
2
(ONO
2
)
3

Hình 1.1. Con đường sinh tổng hợp cellulose ở chủng Gluconacetobacter
1.4. Tình hình nghiên cu màng BC
1.4.1. Tình hình nghiên cứu màng BC trên thế giới
Gluconacetobacter



Gluconacetobacter 
           m. Trong công



dùng l

 

1.4.2. Tình hình nghiên cứu màng BC ở Việt Nam
            
Gluconacetobacter 
Gluconacetobacter 


n cacbon: Ethanol, Glucose, Acid acetic, Agar.

4
)
2
SO
4
.
KH
4
PO
4
, CaCO
3
, MgSO
4
.7H
2
O.


ng: N
2.1.2.2. Thiết bị


Shakergallenkump (Anh).

 20µl - 10ml.

Cân (Precisa XT 320M - 

4
3g
3g
3g
5g
3g
4. KH
2
PO
4
2g
2g
2g
2g
2g
5. MgSO
4
.7H
2
O
2g
2g
2g
0
2g
6. Acid acetic
2%
2%
2%
5ml

MT3 
u
2.2.1. Phương pháp vi sinh
2.2.1.1. Phương pháp phân biệt tế bào chủng Gluconacetobacter bằng
phương pháp nhuộm Gram
Gluconacetobacter   
           
   Gluconacetobacter      


Nguyễn Thị Thùy 15

2.2.1.2. Phương pháp bảo quản chủng Gluconacetobacter trên môi trường
thạch nghiêng
           
Gluconacetobacter  - 5 ngày trong

o
C. Sau 
o
C 
p 

2.2.1.3. Phương pháp hoạt hóa giống


             
ch a
o
C trong

             
m


2.2.4. Phương pháp bảo quản ứng dụng
2.2.4.1. Phương pháp bảo quản màng BC sinh từ vi khuẩn
Gluconacetobacter




2.2.4.2. Phương pháp ứng dụng làm bao bì bảo quản thực phẩm
m: D
: T 
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