


 































Drosera burmanni Vahl 



 
PGS.TS.  
TS. 










:
PGS.



, 



























,




 







 ,  . HCM.





.





















.
, 

 ,  ,
, 

, 


.







 





 h
, 







, 



















 B5 

: NAA (0,2 mg/l)/2,4-D (0,2
mg/l), 

20 g/l, PVP (1,25 mg/l) 5,8, 



.
-  , 













3,03% so 





, 
(2,78%);  2,8% 







 0,78%;






 0,02% ( 






2,4-D (0,2 mg/l), succrose (20g/l), PVP (1,25 mg/l), pH was adjusted to
5,8 before autoclaving.
- Column Chromatography and Thin Layer Chromatography were used to
purify anthraquinone compound in D. burmanni. This compound was
used as authentic sample for HPLC technique.
- Roots of D. burmanni contained 3,01% dry weight (DW) of anthraquinone,
larger than 2,78% in leaves.
- Anthraquinone content of red-leaf D. burmanni Vahl took 0,78% of DW,
compared with 2,78% DW in green-leaf trees.
- Biomass of D. burmannis suspension contained 0,02% of fresh weight of
anthraquinone, while no quantifiable amounts of anthraquinone has been
found in spent medium.

Ho Chi Minh city, September, 2007.
Hoang Thi Thu vi





 ..................................................................................................................... .i




 ................................................................................................................. iii
 ..................................................................................................................... iv


 .................................................................................................. 1
1.1.  ....................................................................................................... 1
1.2.  .......................................................................................................... 2
1.3.  ............................................................................................................ 2
1.4.  .................................................................................................. 2
 ...................................................................... 3
2.1. Drosera burmanni Vahl .......................................................... 3
2.1.1. ................................................................................................ 3
2.1.2.  ........................................................................................................... 3
2.1.3.  ............................................................................................... 4
2.1.4.  ............................................................................................ 6
2.1.5.  .......................................................................................... 7
2.1.6.  ....................................................................................... 8
2.1.7.  .............................................................................. 11
2.1.8.  ............................................................................................ 12
2.1.8.1.  ...................................................................................... 12
2.1.8.2.  ................................................. 12
2.1.8.3.  ........................................................................................... 12
2.1.8.4.  ........................................................................ 14
2.1.8.5. ................................................................................................ 15
2.1.9.  ............................................................ 16

h ........................................................................................................ 17
2.2.1.  ......................................................................... 17
2.2.2.  ........................................................................................... 18
2.2.2.1.   ......................................................................................... 18
2.2.2.2.  .................................................................... 19
2.2.3.  .............................................................................. 19
2.2.3.1.  ......................................................................... 19

 ........................................................................................... 34
3.1.2.3. 3: 

2,4-
 ............................................................. 35
3.1.2.4. 
 ........................................................... 36
3.1.2.5. 
................................................................................ 37
3.2. 







 D. burmanni ............ 38
3.2.1.  ........................................................................................................ 38
3.2.2.  ................................................................................................ 39

in vitro  .............. 40
 ........................................................................................................ 40
 ............................................................................................... 40
......................................... 40
 ............................................................................... 41 viii
ong

 D. burmanni ............ 58
4.3. 
 ............................. 59
 ............................................................................... 59



 ................................................................................... 61

in vitro .................................................................... 62

 ...................................................... 64

 ............................................................. 66
  ................................................................... 68
5.1.  ....................................................................................................... 68
5.1.1.   .......................................................... 68
5.1.2.  ......... 68
5.2.  ........................................................................................................ 69

 ..................................................................................... 70
 ix






TLC : Thin Layer Chromatography (





) x




TRANG
Drosera burmanni  .................. 4
nh  Drosera burmanni Vahl .................................................... 4
D. burmanni ........................................................................................ 5
nh 2.4. D. burmanni Vahl  ........................................................... 7
 ............ 10
2.6. 



Drosera  ......................... 15
 ................... 21
 ........................................... 21
2.9. 















D.burmanni 


5 

(0,2 mg/l) 2,4-






i ..................................................................................................... 52
4.2. 







 D. burmanni ........................................................................................ 57
pm ................... 60
 ................... 60 xi
 ................... 60
 ................... 60


70 ppm ................ 60
D. burmanni 
 ............................................................................................................ 61
D. burmanni 
 .............................................................................................................. 61
D. burmanni Vahl ........................... 63
4.13. 

D. burmanni Vahl .................... 63
D. burmanni  .......... 65
D. burmanni 
 ............................................................................................................ 65
 
 ...................................................................................................... 66
4.17.  ................... 66 xii





 ........................ 18
3.1. Quy t .......................... 39
3.2. 







 ............................................................................ 42
3.3. 









.......................................................... 45
3.4. 







TRANG
 Drosera 
7-methyljuglone ..................................................................................................... 8
 Drosera ................... 9

Drosera ............................................................................................... .13

v 

 ....................................... 33

 ............................................................... 34
ng 3.3. 

2,4-D
 ..................................... 35

 ................................................................................ 36

 ................................................................... 37

in vitro ............................................................... 43

c  ................................................................ 44
  ................... 46

 ................................................................................................ 47






....... 66
1  1






1.1. 
               
, 


 
  .     o  

 

  
 
m .  
               in vitro
3  2







2.
2.1. TDrosera burmanni Vahl
2.1.1. 
 [14]:
.
.
.
.
.
.
Drosera.
: Drosera burmanni Vahl.
burmese sundew.
cburman.

    hay  
 [6], [15], [33], [35].

2.2: Drosera burmanni Vahl [33], [35].

 

h].


    
Drosera burmanni Vahl

     
  Drosera burmanni
Vahl
A
F
E D
C
B

 - 
 
A
D
C
B
6  - 

 
9], [14].




  
N


2.1.4. 
 


 
: S
: C



 - 80%)

 
5 - 35
O
 - 25
O

5
O

 
, t - 


8 2.1.6. 
h 
Drosera in vitro.
 Naphthoquinone
Drosera h plumbagin
-methyljuglone (2.1) [21]. 
    (B
2.2 29].

2.1: Drosera  -methyljuglone [21]
Plumbagin 7 Methyljuglone
D. andersoniana

D. hilaris
D. indica
D. intermedia
D. longifolia
D. longifolia x rotundifolia
9 D. platypoda
D. prolifera
D. pygmaea
D. regia
D. rotundifolia
D. slackii
D. stolonifera, spp. Rupicola
D. venusta
D. villosa
D. madagascariensis
D. ramentacea
D. regia
D. rotundifolia
D. spathulata
D. stolonifera, spp. Stolonifera
D. tracii
D. trinervia
D. villosa

2.2: Drosera [21]
Naphthoquinone  
Droserone

In vivo
In vivo
In vivo
In vitro
In vitro
In vivo
In vitro
In vitro

 
10 hhthoquinone-
Drosera [21].
R
1
R
2
R
3
R
4
R
5

[1]

6
R
7

[7]
CH
3
H
Rossoliside
[8]
H CH
3
2-Methyl-hydrojuglone-4-glucoside  Flavonoid
Drosera ch flavonoid gl
isoquercetin, kaempferol, astragalin, hyper].

Quercetin Kaempferol Myricetin Hyperin
2.5: C29].


C
              




T
  



V               




 in vitro
         Drosera  
Drosera 
in vitro Drosera 