1

Antiinflammatory studies of soot and theformer
plant through TLR4 receptors

Nguyen Thi Tuoi

Hanoi University of Science, VNU; Faculty of Biology
Major: Biochemical; Code:60.42.30
Supervisors: Dr. Trinh Tat Cuong
Date of Presenting Thesis: 2012 Abstract u ngi c
n xut cytokine tiu t c hiu ca TLR4, 
 d c ch cu
ngi cnh c ch yu t t -activated protein
 c ch cu ngi c
o phn u t c hiu TLR4,  
 dng LPS.
Keywords. Sinh hc thc nghi ni cu; Th th; Ch
Thc vt 
Content

I. Lý do chọn đề tài:
ng t   chng lnh t ng
 ngoc bi
bnh. Kt qu ci b hoc triu kiy,
u thi gian tn t chuyn t

II. Mục tiêu đề tài
c chu
ngi cu trong t  th TLR4.
c kh i cu trong t 
th TLR4.
III.Nội dung nghiên cứu 3

 u ngi cn xut
cytokine tiu t c hiu ca TLR4,   dng
LPS.
2 c ch cu ngi cc ch yu
t t MAPK ( mitogen-activated protein kinase).
 c ch cu ngi co phn
u t c hiu TLR4,   dng LPS.
IV. Phương pháp nghiên cứu
1.Tách chiết macrophage từ tủy xương của chuột
T i th a chuc
 c b ng
u ki        u t   -CSF (macrophage-specific
           

5
M 2-mercaptoethanol.
2.Đánh giá hoạt tính độc tố của Wedelolactone tới khả năng sống của BMDM bằng kít
CCK-8
Kh  ng ca t     ng s dng Cell Counting Kit-8 (CCK-8,
     c  c  c

 s gng  
bng phn ng enzyme hi c v   
t s  c
dm mn s t ca mt hoc mt s
lo cn nghiu trong hn hp phc tp.
5. Phân tích ức chế của Wedelolactone trong quá trình tạo phản ứng oxy hóa[17]
c s i Laminarin hoi
c  vi Wedelolacto
t c  vi DHE (Fluorescent Dyes (dihydroethidium) - cht hu
 
2
. Nhng t  c kim tra bng laser-scanning confocal
 nh m u cc x  5

6.Phân tích thống kế
i v lic ly t t qu c lc th hi
-u chnh Bonferroni hoc
Ai vi nhi 

c ch ng P< 0.05.
V. Kết quả nghiên cứu
1. Khả năng sống của tế bào không bị ảnh hưởng bởi Wedelolactone

Hình 13: Biểu đồ LPS kích thích tế bào BMDM sản xuất cytokine tiền viêm và kháng
viêm.
BMDMs t chuc  vi LPS 100 (ng/ml). Dch t c  vi LPS  
nhau l gi, 4 gi, 8 gi, 18 gi, 48 gi s i vn xut
cytokine bt qu c biu hin sai s c lp.
TNF-α
0
5000
10000
15000
0 4 8 18 48 (h)
0
500
0
1000
0
1500

ml)
Quá
trình
tiết
của
cytoki
ne
(pg/m
l) 7

3. Wedelolactone ức chế quá trình sinh cytokine thông qua thụ thể TLR4 kích thích bằng
LPS
Hình 14: Biểu đồ Wedelolactone ức chế quá trình sinh cytokine tiền viêm trong BMDM
được kích thích bằng LPS.
BMDMs t chuc  i Wedelolactone   

15000
DM 2.5 5 10
20(µg/ml ) 0
500
100
0
150
0
DM 2.5 5 10 20
(µg/ml )
INF-ү

0
2000
4000
6000
8000
IL-10

P-ERK
P-p38
LPS
0 5 15 30 60 120 (min)
LPS 9

c  vi LPS (100ng/ml) ti m t 
t  nh  

6. Dầu ngải cứu không gây độc đối với BMDM

VI. KẾT LUẬN VÀ KIẾN NGHỊ
KẾT LUẬN
1. c ch n xut cytokine tic ch 
i n  th 
bng cu t c hiu LPS.
2.   c ch     a yu t t    
(extracellular-signal-regulated kinases
3.        ng c ch     a
p47phox.
4. Tinh du ngi c c ch n xut cytokine

1. 





, , 



, , 


(2009), 















 (Premna

















 (Sapium
sebiferum) 



, Tp ch Dưc hc, 50(411), tr. 36-39.
8. , , 



, 




Tài liệu tiếng Anh
9.            
Nature, 420, pp. 515-516.
10.      -like receptor rignaling: emerging opportunities in
human diseases Current Immunology Reviews, 1, pp. 81-90.
11. , Meakin C, Millet S, Gardner CR -induced
serum TNF-alpha prGeneral Pharmacology,
31(2), pp. 301-6
12. -1: a signaling non-TLR pattern-Nature, 6,
pp. 33-43.
13. f Angelica sinensis
ethyl acetate extract and major compounds on NF- -activation activity and LPS-
induced inJournal of Ethnopharmacology, 129, pp. 244249.
14. Chao W and     Isolation and identification of bioactive compounds in
Andrographis paniculata (Chuanxinlian Chinese Medicine, 5, pp. 17
15. -
         Molecular
Endocrinology, 17, pp. 860869.
16. Cohen J  Nature, 420, pp. 885991.
17. Cuong TT, Yang CS, Yuk JM, Lee HM, Ko SR, Cho BG, Jo EK Glucocorticoid
receptor agonist compound K regulates dectin-1-dependent inflammatory signaling through
Life Sciences, 85, pp. 625-633.
18. 
and anti-inflammatory activities of Sargassum swartzii (Turner) C. Agardh (Phaeophyta) and
Ulva reticulata       African Journal of
Biotechnology, 10(12), pp. 2308-2314.
19. 
 European Musculoskeletal Review, 4(1), pp. 24-25.
20. Ferwerda B, McCall MB, Verheijen K, Kullberg BJ, Van der Ven AJ, Van der Meer JW, and
       -   

-inflammatory function of arctiin by inhibiting COX-2 expression via NF-
Journal of Inflammation, 8, pp. 16-24.
30. 
regulatory gen cassette spatzle/toll/cactus controls the potent antifungal response in
Cell, 86, pp. 973-83.
31. Medzhitov R, Preston-
Nature, 388, pp. 394397. 13

32.           -like receptors for
Pharmacology Review, 61, pp. 177197.
33. Paur I, Balstad TR, Kolberg M, Pedersen MK, Austenaa LM, Jacobs DR, Blomhoff R
           -kappaB in
Cancer Prevention Research, 3 (5), pp. 653
663.
34. tor-kB- A pivotal transcription factor in
   The New England Journal of Medicine, (336), pp. 1066-
1071.
35. ”, International Journal
of Pharmaceutical Sciences Review and Research, 9, pp. 1-7.
36. -ruses
activate B cells via interaction with toll- The Proceedings of the National
Academy of Sciences of the United States of America, 99, pp. 22812286.
37. Riedemann NC, Guo RF, Ward PA treatment of sepsis”,
Nature Medicine, 9, pp. 1724.
38. 
            
glucocorticoid receptor agonist for the topical treatment of in  