1
TRƯỜNG ĐẠI HỌC NÔNG NGHIỆP HÀ NỘI



Chủ đề 9: Phân tích khả năng chuyển đoạn DNA tái tổ
hợp của sinh vật chuyển gen ( Động vật, thực vật và vi
sinh vật) sang hệ vi sinh vật ở người và vật nuôi
 !""#$%&!'(
Nhm sinh viên )*+
,"!-#. //01,1
2"3&45 //01,6
7"89&45 //01,/
1"4'&45 7701,:
" 8;<.=>
?@<ABCD<EF=GH<A<A<F94I<-*@3>#E.<@DJ"
2
?K#EF<@L-@M>NOP@@Q<@B@L-@R=SC<T*U
<AMT@@CD<V@9=;@EI<N9CW@VXH@M=-N9*SY#-<5*9=Z#@L-
@@9C[@<A<F"
I-$@)A.<>#\VF<A@>#]-$Y#-.=>N9NI#@@CD<@#$KH
@)<K@#$K^H=)9IH44_M9D<#U-$VU`49@R@)(
_<F9`
" a#
," b#<Ac@#$KBJd9CD<
,", A<?
'@FI<OEFBJ
FE9V#e@)<K@d]5*DJ9N9*a<-=B<f<A_@L-<F
E9T<AE>*;<@L-<FE9@)II^=K*<FJD=;@EI<[N9$F#<SV(BJ"
gF#<S9$@)V(f<FJD=Bhd<i*U<AME9=K=-9*U
<AME<AV#eTM=)C($A-C?<<GOJ"

#$T@)<K5$A-C?#BJEq]zN\)-@.<-$<B^=>#VI.<
=&@C?<f<A_@L-<FE9"]zN\<FE9y"@N@-]k=a@-T@R<A_
<9V(BJ9EFBJ@@JN-C*<?@I@)I#Y#("
^<FE9#BJ<AE>*;<@)@@5<S#BJm@*JH<H@Hr-@<An"R
={=O@<CB@*a<JZ9@W#_>#NBV#e"u<AHJ<@@@#Cm<A@=5$[
N9dJN@@@#CnJH#*-H={<A_<9#BJ@)70=F:0=K*D<A<FE9@)V(
f  h    d  *B@  V|J  Z#    @L-  E.<  Vc  #k  9"  l;<  V@T-H*JN#C
rN#Ht-H@)*a<CSNOB@F<i1=F:=K*DmAH@HJ<ACnT*9^=K*D9$
<A@<DEF<d*B@V|J@)@@@;JE-t'<Ac<?C-# /}j7}
=O@[N9~=K*.J<•€m#J<-VHC<Hn"?VIN9@@=K*.J<•9$=;@EI<@#_d
@L--H*JN#Cm<AEaH@)V(600=K*D$n9<'=SF*_d@L-@@
N9V@={(<P@cC--H*JN#C@sEFBJB@@V#e<AN9"
!ZN@@V#e@s#BJ<A*a<-=BB@L-@#<AcCS"
A-=B#BJT<FE9<GOJ*a<-$>#JA<H=O@[N9~@@5<S#
BJ€T@REF=G*9<FE9=K@)<Kh=BdBN-"D$T@@=K*<•
<K@sII<A-=B#OJ"
m7n P@<@=Bd
4
AEFBJTV#eV(BJh<#D&@d"@<FE9V(BJ@)<K
hd>#'@(,000NZC<FE9VUV(BJ"
8>#VIY#-<A[<W-=K<?@I=O@EFBJN9dJ(@)*B@V|J9
=BEFBJJ(@)<A[NOJ5<z<S<K#N9100"000-N<mNV(,o200EaH
@L-V#enT•=5$VUJ(N9B@-.<"SNO<FE9=O@EFBJ
m<A-CrA*-<Cj<KEFBJn<f<sNI<#Dk=a@L-d@=FNR@*9@@=K*<•
<KmAH@HJ<A  C<HCn  E{ 3-    @@=B dh9m<MV(,0=Bo<FE9n"
u<AHJ<@@@#CJH#*-H*p<FE9@)<KhV(,0J5<zd*B@V|J9,0‚
,/VEJmVNE-t'J-ACnoJ5<z"d=O@.J<•9<FE99E&Ht$*@h<N9*(*<A[
NOJ5<z@3V(:VEJoJ5<z9*B@='"
8>#<R&N9<AEFBJ_-H*JN#CrN#Ht-HT=BdJ(@)@#pV\<?
,,EJ@#$EI<=K]($A-Y#<AchVU=(O@9<#Dd"#p9$=O@

8&•- [V#e=MA#a<E-k*@@<A?@V#eA-*5*T@)<K5$EI
;@VUTV\C_=M<#@L-M9=aD<T@)@#^<P@.<C-#
jF#VP;@VˆVP<#x<I"
j!<<AK<S<<A@@*U<AM5<B<U<MTVUC-E9"
jM@)NUY#-<5m*a<CSP<VU@)NUHNN-TNHECHNN-n"
j*H=MN#@t-
j#$K<A-<<9<A<
jU@)]$-CH
d?-9<P@.<V#$9<P@.<CD<[@TvHAH$@-[V#e
=MA#a<<9,7S@P
Escheriachia Serratia
Shigella Proteus
Edwardsiella Providencia
Citrobacter Morganella
Salmonella Yersinia
Klebsiella Erwwinia
Enterobacter
A@@SV#e=MA#a<VK<AT@@S@)\•-<A‰[@.<N9
Escherichia, shigella, salmonella, klebsilla, proteus, yersinia.
6
-@<E-@@NN#CvrE-@NHA#*NC<A#*y"@N
A=M<#)-@L-*a<MEc<M@)<9<Af*c<sV#eV@
-#TE-k*V#e@)NO9V#e@)B"@V#e9$<B*a<<F@5Eq
.<=&TRJ=(*E(@B<=aCN\@L-A#a<9@L-@'<KT=(*E(@@'<K@S
*[V#e5$EI"
A3,2j21C-#VCTS<#)-@L-<AŠ9<9UV#e"<AŠ<FJ
]R@*U<AM]#Y#-Tf#ST@@V#eE9*]5*DJY#-*IT
*QT[T<A?@<A9c<9*a<IV#eE-k*@@V#e@)P@9V#e@)B
<A=M<#)-=O@[N9V#e@P=MA#a<"
IV#e9$_MNE-k*20<AI#V#eVP92<sV#eVˆVP

<AV-*IB9$TA#a<;@=F@@V#e@)<A<W@f@R<-"
@HVVC=O@Cz•N9*H@[N[@H nptII (neomycin
phosphotransferase II) VV--*$@ŽH hygB (hygromycin phosphostransferase) V
8
$A*$@v9H streptomycin phosphotransferase VC<AHJ<*$@ŽH amp
r
 V
-*J@NNŽH@
A
V<H<A-@$@NT""
A*a<E9E@)=>@DJ=F#$@'5$MVCVCz•<?@Je
EF=GH"
~4I@Cz•l@)<K<I@<f<PVVC@L-V#e"Z#F<
@@CD<EF=GH@)@W-@@HVC@)V(f@#$KC-V#e5$B
@M"
#$@-<KV•=&^(_@L-<?@Je*EF=GH5$&WT=a@
<STVVCT*a<CS@W#Z=5$@<.$Aq@#a<fUEF=GH
@)I<O-E&C"
4I@Cz•@@HVVC@QN9*]($A-@@#$@'@<?@Je*EF
=GH"M<-=;<A-@5#XNI#@)<K]($A-C?@#$K@@NBHVVC<i
D<NI#<A#$>@L-@5$-$<?@Je*@#$KH9<AH@L-@@V#e<AA#a<
-$VU`F##$@'9$N9@)<D<<cI@Cz•<?@Je*@#$KH@)<KN9*]#.<
I@@@LV#e@)V(fVVCT=)N9K*[-=SCW@VŠ@a
=k"€  j  <<J oo-<A"@*"oC#@jVHo<#@jJ-*jEHjjHj-$jjV-jCj
,1+†:0"<*
D$.=>@ZY#-<5*N9V(f@#$KH9ICD<-$VU`
1" !5<P@V(f@#$KH9ICD<=MA#a<@L-M9
=aD<
1", Od<A=M<#)-
M<M<#<•<S<K#N90",=F,do9$<A@F=af@L-*c"

=O@c<9<iH9$E&E.<B<-@)E_-@B9$9E&J5L$E_@@
Ht$*H<#)-T=)V(fVVC=O@=-9@'<KE&E.<B<Ht$*H
H<J=O@<BA-=O@NBEX"la<=>#=Y#-<5*=)N9HntpII<k<BJGEF<A
<?E_c)@)#kS@<iV#ey"@NT9)NY#-=F<PV=SN-
VCP<=O@Cz•N9*O@Je*@M9<R\"
lD<=ad<S<K#=K@)<KJ<IEFBJN90T0,o*NT*a<@CS<.J=Fp
VU<KJ<IEqJ'JJ)-[@"
10
D$>NOd<A=M<#)-<k<B<A*a<<M-.<=&=L=K
EFBJ9V#eA.<P<9Z#VU@)"
1"2 .#<AR@TVP@<@=Bd
?@<FE_c<.<@(@@d=>#@)@ƒ@.#<AR@)-[@TD$T<.<@(^#$
@'<>*eNY#-=FI@<#<•dC‡VUJ•<#a@9#kS@N9*9J•
<#a@9<Ac<?@L-)TD$V(fEFBJ@L-<.<@(@@d<A<?@Je*EF
=GH9<A#$><S9ICD<=MA#a<N9-#"v@B@=)T@)>#$F#<S
BI<O@#$KT.J<#9C?G=&@L-d<AV#e"?J‘@.#<AR@
d<AC#S<Y#<AcEF=G<W@f<A@V=O@<#)-9<AY#<Ac<#)-T
@QC?@)*;<@L-@@Ht*$HB@L-V#e@)-<A3Y#-<A[<AC?J5
@h<dB"
'^-V#e@)V(f*-9h=BdB9<AIH@L-
@RV@@=Bd_*B@<•9=XXC?<'=k@-><Ac<?;@9<9
^ƒ<E(=a@NDJ"v@B@=)TC?EK#I@L-@@H*@#$K9=3XC?@)
*;<@L-@@<Ac<?JA*<A=>#3-<P@OJ"#S@ƒ=K#$<Ac<AY#Z<KV#eT
@@<P<ABJ(*-#<F@B@<A-"
I<OEFBJ@s]($A-@@=Bd@)<A[NOJ5<zi-J(T<i
,06‚,0†-N<"@=BX',0/;@N',0:=>#VU@)V(fEFBJ"
D$T(Cz@3<k<BNOd<AI<#)-TVP@<@<F9`
I#@)q*<A=B,06j,0†-N<`…@]#.<J5@h<=R=BH=)VP@<@
D$N9A.<<.J"
1"7 (f#BJ@L-<FE9V#e

j l#9*U•–<@@F<d•–!••–=I
j d&@B@XTV#e•–<@@F<d•–!•Eq*kA$,TA$2TA$7T
A$1
m1n F<Y#(
U@)d<<GOJ=O@J<I<A<AE.<Vx*#*UT*#N.$<i
@(2)*9"AVdV#e=O@J<I<A&@B@X9*#*U
<AM#U@.$_<.<@(@@<M=K*TVUJ<Id@L-U@#$KH
9@(U<A#$><S"
"F<N#D
12
4I@J<<-d<<GOJ<i<W@f9@'<K=aD<VU=O@J<
I
…@C#.<@#$K9<GOJ*a<@@@)@W@f@L-d<i<?@Je*<#)-E_
@@<FE9@L-ICD<=MA#a<9@MN9Z*W@0
l—
," #$%&!'(T#$%&ƒ$"5$<Ak@UIC[@T20,,
2" ""˜#$%5dQT!"!B*9G"vFBJT20,0
7" #$%v"4CD<[@=B@'T2001
1" l-CC*A-E-Nt-jl-A#@@"<AHHj$H-AN<#-NC<#$<HHrrH@<Cr-H<
@<-HH<@-NN$*rHv<,†6*-tH<HH-N<C<-<#C-JHArA*-@Hr
CHHJT200†
/" -NH#"