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Contains a signal
sequence
+ A,B, C sections of
protein
Signal sequence is
removed after targeting
to RER
Translation continues on
RER (forming
proinsulin)
Removal of 33 amino
acids at Golgi
and S-S joining of A
and B chains to form
insulin
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Human Insulin Production by Bacteria
6) join the plasmid and human fragment
and cut with a restriction enzyme
Human Insulin Production by Bacteria
Mix the recombinant plasmid with
bacteria.
Screening bacterial cells to learn which contain the human insulin gene is the
hard part.
Route to the Production by Bacteria of Human Insulin
A fermentor used to grow
recombinant bacteria.
This is the step when gene cloning takes place.
The single recombinant plasmid replicates within a cell.
Then the single cell with many recombinant plasmids produces trillions of
like cells with recombinant plasmid – and the human insulin gene.
One cell with the
recombinant plasmid
Route to the Production by Bacteria of Human Insulin
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If adult suffers with hypopituitarism,
HGH therapy is also available
as normally payable under an insurance policy.
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HGH could be purified from cadaver (tử thi) pituitary glands
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Creutzfeldt-Jacob Disease (CJD) is one of the risk of using brain tissue
24 cases reported by 1993 in France from cadaver HGH
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Native human GH contains signal peptide
needed to direct nascent polypeptide to RER in
eukaryotes