ứng dụng công nghệ gen trong y học và chẩn đoán - Pdf 24

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sequence
+ A,B, C sections of
protein
Signal sequence is
removed after targeting
to RER
Translation continues on
RER (forming
proinsulin)
Removal of 33 amino
acids at Golgi
and S-S joining of A
and B chains to form
insulin

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Human Insulin Production by Bacteria
6) join the plasmid and human fragment
and cut with a restriction enzyme
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Human Insulin Production by Bacteria
Mix the recombinant plasmid with
bacteria.
Screening bacterial cells to learn which contain the human insulin gene is the
hard part.
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Route to the Production by Bacteria of Human Insulin
A fermentor used to grow
recombinant bacteria.
This is the step when gene cloning takes place.
The single recombinant plasmid replicates within a cell.
Then the single cell with many recombinant plasmids produces trillions of
like cells with recombinant plasmid – and the human insulin gene.
One cell with the
recombinant plasmid
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Route to the Production by Bacteria of Human Insulin

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If adult suffers with hypopituitarism,
HGH therapy is also available
as normally payable under an insurance policy.
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HGH could be purified from cadaver (tử thi) pituitary glands
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Creutzfeldt-Jacob Disease (CJD) is one of the risk of using brain tissue
24 cases reported by 1993 in France from cadaver HGH
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Native human GH contains signal peptide
needed to direct nascent polypeptide to RER in
eukaryotes


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