Khóa luận tốt nghiệp Viện Đại Học Mở
MỞ ĐẦU

 !"#$!%&#'()*
+,-  ./&01* 23
4/52! 4-  .4!/67
# 8*9:.4(;
8<=3 >/!
?@28A(
B36#C$8*9:, .
D2(E ?@28A*+?
& C!(F*#$
!%#<<G?&5@28A
@2$H/?IC$0(
@28A .>/D
2#5!@2H<<G#28A!C
 8C$<=(; 2@2.
D2*$&.8A(JC$! .8A
K.L! !.M ! <:.) / !.
;:! :N; 4.;:! :!23 ! .
$<=4#"34 .>/4#".
/O 5.2P/.AN
 JQ#",.8A! :R2:8
<= !S#TD268#8<=
;:!:!:R2:<=?IC;:! :!4$
UD5!A+D5*&CH<
<G(E #768?IC;:!:!:R2:<=?
IC;:! :!VV&(W>/6H.F2
,&7&@2+" 604 (
Khoa Công Nghệ Sinh Học Vũ Thị Hằng - 0604
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6P,Ek[/ i2234/"
(;"&: 2:2=6#Ek?m#: n&#
P+ .23! oR2:&(; $.
m>/4#"#7/7 nEk: 2:&/jEk
0/QmEkn,2fk=(Ek[+*
fk.I-,:R2: Om:#:::
p<::<:Ekp !2::n(Ek#: !23!2< >
:m&#72<X:!#*@n!2<;,#*@
Agrobacterrium tumefaciensm&#72<X:!&/ #7n
!Fmretrovirus >andovirus*/&#72<X
:!&/ 3#7n(Z"8!#7Ek[# 0
#72<X#/"&!C#72?IC
m&C#&n?@22=6(
Khoa Công Nghệ Sinh Học Vũ Thị Hằng - 0604
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Khóa luận tốt nghiệp Viện Đại Học Mở
 .PQ23! :R2:&m.!:R2:V
<^n Ek2=6# Ek#: mUP232
,2#XI P*Ong65!6*&< 0
/Q1V<^i!:PV*(a&D? q
5[,#: #"23 >2342?Ek,
: 2:2=6m/6?@2*2 24#: 
*O#X#: [#"#2?Ek*[
 >[n(;C??@2 q$3$
#"#72 <X#72C 2
.<<G&4!mC*4#"#*@
C&4#"&/ 3#7#7n(; $*U
&/ #72#: 4(r0< !2</H:R2:&
P #X23#H^IH 23:$*233^
7/&*!2<[2C*?A ^1

$T>! .# 5?0V#":
<2"!6D"8*9:,.#w2.(J
82^#,yLz@24#" .T
,7*?A!$:*4m,l!2<#: n
 7 #*@6,0m#^<= .3n
.#3#7(JA2! i$*4<9#
4</<g&!2<=<#& 1
9<5#7*4#*/{!230
2"4#"(;00#7yLz!C
#76{!C #<g&<,$! 
#77D?62&2.N
I.2 Đại cương về vi khuẩn E.coli
;: ' FS  l" mYdh]n|  ' tETmYdh}n 
*@ Escherchia Coli   6  *     ! Bacterium Coli Comunis /5
.E.coli-1V,3m3#V4,3 n
^*1<<^"3 ,3#7m'FS
l"Ydhen(;U!E.coli1*&!~e(]•~~(]•2
!]d(]•E.coli IC#413#7U#.*#
Khoa Công Nghệ Sinh Học Vũ Thị Hằng - 0604
5
Khóa luận tốt nghiệp Viện Đại Học Mở
i&* #7&(t A#AH?$E.coli0! A
9(;: a:x2,E.coli.>#$&
): :6(; 4234: :#XD
 #/ .#3#7(
I.2.1 Đặc điểm hình thái
E.coli!! *@57PVX!<3
*5/ 2/P2y22.g21
V1*^"#*@\p]LI€(_p€(}2(; 0
-#*@5V86!•*I&qP(

l?8< !mƒn?8Flm‚n?8Lfmƒn?8r\)
mƒn 6„::mpny:!mpn(
I.2.4 Sức đề kháng của E.coli
E.coli/H6<13~~

J 23.}€

J ]€-
mv!! #L!:Ydd~nm'#Sl"Ydh]n(JCQK
kI:J! ,M 2 !6<E.coli ~-(
I.3. Tình hình sản xuất và ứng dụng enzyme trên thế giới và Việt Nam
I.3.1 Tình hình sản xuất và ứng dụng enzyme trên thế giới
oR2:#X#QD*UI- ?
8I? 442**944
-#gI- ?8 &/ m n(Z1#7
:R2:(0/?#$:R2:!0
1688<=:R2: '(
oR2:.<=: K*:R2:
*96!2 $*7!  3,234:R2:
… 6!-CQ 6
!: "2 24(r >:R2:*96!1<
&@2*V&(oR2: 3+/C*†
0 D 4  ( F^<= :R2:  0 2     ? IC +
Acitinoplanes"Zymomonas(;+#Hmn&3,P(
E 7<=i6!:R2::R2:<Q
:R2:&/&! 6!* ><=# 
2=^*(rA2:R2:02?IC 
023+C224#C22:DY‡]+#*@X!e•+
Khoa Công Nghệ Sinh Học Vũ Thị Hằng - 0604
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Khoa Công Nghệ Sinh Học Vũ Thị Hằng - 0604
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Khóa luận tốt nghiệp Viện Đại Học Mở
m\(Y~€(€€€C‡A2n#I ! :px :~~•mY(_~€(€€€C‡A2n(
lV!":R2:<=1283$3! 
:R2:0CI- ?8,(a ?h€•&@2!
:R2:,<=,0C6(
J ::!:R2:<=$C<Q 
D5=?ICx 2!22$2H?ICC@
3<( ;&  & !:R2: 2  r</  k2!:
J:!!! :l::N)<=$ &/&@28A
-<?ICC#/3C(
; @2&@2:R2:<=#"
_2=^^K
pW$U*&2*&,6!(6!,
@2!?@28i4+3#7#
#75D$ [(E 
V6!=3# &4/6 4! #
&4/6 $**^7(J&4!2
[V6!#(; .
&@2:R2:<=23=!CV&=(; 
&/&./3/2†/N./[
k2!:/Q !:R2&,6!T4(; ?IC
x 2 :R2:!: # :: <=&:R2: +
6!/H# Q(
p;28<=$C ?IC/
/D:x 2"4N :: O?^!,/#
^A[H,13C(k2!::::2:!!!:
 $* #&#!"D?(l ::^!2
2$2<• *!:?31,/3 #3X,/D:(

)@2&@2:R2:<= 
<@2&&/&8A-(W>
#<=:R2:,##7 "? 
Khoa Công Nghệ Sinh Học Vũ Thị Hằng - 0604
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Khóa luận tốt nghiệp Viện Đại Học Mở
I!%2.68*<=(
; <#<=:R2:<=+
A2\€,&*‹\€(oR2:+"&L!#l::<=
Ti(A2YdhY<=:R2:k2!:,Z!!/!Ti1
 3  - P. (  oR2: y!:  l::  o:
l ::<= D5Cj2! /9V
*?J:!!! : (
; 3<D5<=:R2:!!
#!22$2<(.<=:R2: !!::2 
/ I:<:C.#,!6*&  !!:.
#7!*9<#162$20(
; /3>#@&@2:R2:
<=! ::!::!!! :2!: I< :<:A#74#
D?D5@(L34:R2:/[I# ! I
X#*:2<G<4A/ 2:! !!::!:N
:R2:<=@@#&/@/26<*4i
.!22H#A3i <(
; :R2:<Q?IC8A 3#7
j2AH8AA4<= 8A(J:R2:
<=#"2=^!:R2:,2!: ::>
!:!!! :#:2:!!! :(J-,C!"
<<'C=0.!2AD?<=8A(
W$%SD*@/H8A 3#7X
 #546,-^#"#A! 8

7:68 :m:< !::n<=:R2„::
H!: "N
J &.#g jdd•*? A8<=,
:R2:#g1<$2A(;601680/?#$C-
#0&[:R2:21#,:R2:K
p;[:R2: KC ^0:R2:
[/j . &*&#[CPC
Khoa Công Nghệ Sinh Học Vũ Thị Hằng - 0604
12
Khóa luận tốt nghiệp Viện Đại Học Mở
0?I #&*&2I-C**A(W&23#
:R2: <=< !!2 ^
0::(
pZ&[C-:R2:K<=*b7<$!2/&[
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 :?/&2 3,:R2:2=^!2
  ^ 0 >:R2: ^2"(
poR2:<= ^@ $H(
pZ? #2.K<=:R2:I!%C? #7!
<=#7!&(
I.3.2 Tình hình sản xuất và ứng dụng enzyme ở Việt Nam.
 F68#8<=:R2:1F2U2"7
-% #7*‹1!(J &F223
:R2: ?IC1D222"U<+!1D
292#X^2,.#6
8(oR2::+<</Xm;.WrZ* r3n
/ 2:!+<8mWrˆ4yr3np2!:! :2!:
 ::+##7m#@2n
&@2:R2:<=3C1"
!2!:,&<Q ?IC/.