BO CONG
THLTONG
TONG CONG TY
GIAY
VIET NAM
Vien nghien
ciru
cay nguyen lieu giay
BAO CAO KET QUA DE TAI
CAP BO NAM 2009
De tai:
NGHIEN CUtJ LAM CHU CONG NGHE
VA XAY DUlVG MO HINH CONG NGHIEP SINH HOC
• • •
SAN XUAT GIONG VA
RITNG
TRONG CAY
NGUYEN LIEU GIAY NANG SUAT CAO
Cc
quan chu quan: BO CONG
THlTONG
Co^
quan chu tri: VIEN NC CAY NL GIAY
Chu nhiem de tai: ThS. Pham Thi Kim Thanh
PHII
THO, THANG
12
NAM 2009
-,7/
j
JJQA

Trieu Hoang
Son
PHU
THO,
THANG 12 NAM
2009
MUC LUC
Thit tw Noi dung Trang
Danh muc ky hieu va cac chir viet tat ii
Danh muc bang va hinh iii
Tdm tit iv
I. Tdng quan 1
1.1. Xuat
XII
cua de tai 1
1.2.
Tinh cap thiet va muc tieu nghien
ciin cila
de tai 2
1.3. Ddi tugng va ndi dung nghien cuu 3
1.4.
Tdng quan tinh hinh nghien
ciin
trong va ngoai
nude
4
1.4.1. Tinh hinh
ling
dung ky thuat nuoi cay md va giam hom Bach 4
dan trong san xuat lam nghiep.

ciia
cac ddng Bach 21
dan nghien cuu.
2.3.4.
LTng
dung cdng nghe nudi cay md va giam hom trong san xuat 24
cay gidng Bach dan.
2.3.5.
Xay dung va cham
sde
vudn vat lieu gidng cac ddng Bach dan 29
2.3.6. Kha nang sinh trudng tai cac md hinh rung trong. 30
2.3.6.1.
Kit
qua sinh trudng cua rung trdng tai Lap Thach - VTnh
Phiic.
30
2.3.6.2.
Kit
qua sinh trudng cua rung trdng tai Tam Thanh -
Phii
Thg. 33
III
Kit
luan va kien nghj 36
Tai lieu tham khao 38
Phan phu luc
DANH MUC KY HIEU VA CAC
CHlT
VIET TAT

He sd bien ddng
cila
chieu cao
11
DANH MUC BANG VA HINH
Bang
01
Kha nang sinh trudng phat trien chdi Bach dan in vitro
18
ciia
cac ddng nghien
ciin
(sau 4 tuan nudi cay)
Bang 02 Chat lugng chdi Bach dan in vitro cua cac ddng nghien 19
cuu
Bang 03 Kha nang ra re in vitro
cila
chdi Bach dan (sau 2 tuan) 20
Bang 04 Anh hudng cua thdi vu den kha nang ra re
ciia
hom 3 ddng 22
Bach dan (lin 1- thang 3/2009
din
lin
10-
thang 12/2009)
Bang 05 Chat lugng cay hom
cila
3 ddng Bach dan 23
Bang 06 Sinh trudng cua rung trdng 04 ddng vd tinh Bach dan 25

nguyen lieu gidy (FRC) nghien ciru di tdi "Nghien ciru lam
chii
cong nghe vd
xdy dung mo hinh cong nghiep sinh
hgc
sdn xuat gidng va rwng trong cay
nguyen lieu giay ndng suat cao". Ndm 2007 - 2008 de tdi dd hoan thdnh cdc
ndi dung sau: Dd xdc dinh vd
lira
chgn 4 ddng Bgch ddn uu trdi, ndng sudt cao
da dugc cdng nhdn gidng (giai dogn 2000 - 2006); Nghien ciru nudi cay md vd
gidm hom cdc ddng vd tinh Bgch dan da dugc cdng nhdn gidng:
PN3d;
PN116;
PN54;PN46. Ndm 2009, sau 12 thdng thuc hien vdi su cdng tdc
ciia
Phdng
nghien ciru nudi cay md, Phdng Lam sinh - xd hdi- mdi trudng, de tdi da hoan
thdnh nhirng ndi dung nghiin ciru dgt ra trong ndm 2009 cu the nhu sau: Dd
ddnh gid khd ndng nhdn gidng in vitro cua 04 ddng Bgch dan nghien ciru vi
khd ndng nhdn nhanh vd sinh trudng cua chdi in vitro, khd ndng ra ri cua chdi
in vitro; Ddnh gid khd ndng nhdn gidng bdng hom cua 03 ddng Bgch dan
nghien cuu; Xdy dung vudn vat lieu gidng Bgch ddn vd 02 ha md hinh rimg
trdng Bgch ddn; Ung dung ky thuat nudi cay md
—
gidm hom Bgch dan theo
hudng cdng nghiep: tgo ra
20.000
cdy gidng.
IV

Kit
qua
din
nam 2006 cac ddng PN2 ;
PN14;
PN3d; PN46; PN47;
PNl
16
da dugc bd Ndng nghiep va phat trien ndng thdn cdng nhan la gidng dua ra
trdng rimg cdng nghiep d viing Trung tam va cac viing cd dieu kien sinh thai
tuong tu. Nhiing ddng Bach dan dugc cdng nhan gidng PNl 16; PN3d; PN46
cd nang suat cao, song hien nay chua cd nghien cuu cu the ve ky thuat nhan
gidng va lam ehu cdng nghe san xuat gidng cho timg ddng.
DI
gdp phan nang cao nang suat rung nguyen lieu giay, nam 2008 Bd
Cdng Thuong giao cho Vien nghien cuu cay nguyen lieu giay (FRC) nghien
Clin de tai "Nghien ciiu lam
chii
cong nghe vd xdy dwng mo hinh cong
nghiep sinh
hpc
sdn xuat gidng va rwng trong cdy nguyen lieu gidy ndng
sudt
cao".
DO
ban che ve kinh phi, Hdi ddng khoa hgc
ciia
Vien va Vu khoa
hgc cdng nghe cua Bd nhit tri gidi ban dk tai tap trung nghien cuu vao mot
loai Bach dan E.urophylla vdi cac ddng PN3d, PN46, PN54,

"Nghien ciru lam chu cdng nghi vd xdy dung md hinh cdng nghiep sinh hgc
sdn xuat gidng vd rimg trdng cdy nguyen lieu gidy ndng sudt cao " dugc thuc
hien.
1.2.2. Muc tieu nghien cuu ciia de tai.
* Muc tieu tdng quat:
+ Lam chu cdng nghe nhan gidng md-hom va ung dung nhan giong
mot sd ddng vd tinh Bach dan uu trdi, nang suat cao da dugc cdng nhan gidng
(giai doan 2000 - 2006): PNl 16; PN3d; PN54; PN46.
+ San xuat cay gidng vd tinh Bach dan.
* Muc tieu cu the ndm 2009:
+ Xac dinh kha nang nhan gidng bang ky thuat nudi cay md va giam
hom
cila
cac ddng Bach dan nghien
ciin.
2
+ Xay dung md binh rimg trdng Bach dan.
1.3. Doi
tugng/pham
vi va noi dung nghien cuu.
1.3.1. Doi tuong nghien cuu: Cac ddng vd tinh Bach dan urophylla da
dugc cdng nhan gidng cip qudc gia: PN46; PN54; PNl
16;
PN3d (giai doan
2000 - 2006) dugc dan gidng tu rung trdng thi nghiem cua Vien nghien cuu
cay nguyen lieu giay Phu Ninh -
Phii
Thg.
+ PN3d: La ddng da dugc Bd Ndng nghiep va Phat trien ndng thdn
cdng nhan gidng Qudc gia nam

Xay dung vudn vat lieu gidng Bach dan.
3.
Xay dung 2 ha md hinh rung trdng Bach dan.
1.4.
Tong quan tinh hinh nghien ciru trong va ngoai
nude,
co sd ly
thuyet.
1.4.1. Tinh hinh ung dung ky thuat nudi cay md va giam hom Bach
dan trong san xuat lam nghiep.
Hien nay nhieu nude phat trien da thuc hien kinh doanh rung nguyen
lieu theo phuong thuc dau tu tham canh cao. De cd cay gidng chat lugng tdt
phuc vu trdng riing, cac
nude
tap trung vao viec san suat cay con theo phuong
phap nhan gidng md - hom, cdng nghe nay dugc nhieu noi tren the gidi ap
dung de nhan nhanh nhung ca the chgn
Igc
tao ra cay con chat lugng cao dap
ling dugc nhu cau trdng rung d qui md
Idn.
Nhimg rimg trdng bang cay con
tir
md-hom da dat dugc do ddng deu rat cao, duy tri dugc nhirng dac tinh uu
trdi
cila
cay me (sinh trudng nhanh, nang suat va chat lugng cao).
Song song vdi viec chgn
Igc
nhimg ddng va xuat xu tdt, viec nhan

Vao dau nam 1950, Bach dan (Eucalyptus) van dugc coi la loai cay
khdng the nhan gidng bang hom, nhung vao nam
1961
Giordano da giam hom
thanh cdng bach dan
E.camaldulensis
mot nam tudi vdi ty le ra re dat tren
60%.
Nam
1963,
nha nghien cuu ngudi Phap la Franclet da dua ra mot danh
muc bao gdm 58 loai bach dan da dugc thu nghiem giam hom va dat dugc
thanh cdng d tung muc do khac nhau. Dau nhiing nam 1970, cac nha khoa
hgc Phap da di sau nghien
ciin
tim ra quy trinh cdng nghe san xuat cay hom
Bach dan cd nang suat cao tai Trung tam ky thuat lam nghiep nhiet ddi
(CTFT) Cdng gd. Cdng nghe nay da dugc mau chdng ap dung vao san xuat
lam nghiep d Cdng gd, dac biet la viec san xuat hang loat cac ddng vd tinh uu
viet cd ngudn gdc tir cay lai tu nhien hoac cay lai nhan tao. Trong sudt 10
nam, tu nam 1978 den nam 1987, Cdng gd da trdng dugc 25.000 ha rung
trdng bach dan bang cac ddng vd tinh cao san. Cac ddng vd tinh ndi tieng bat
ngudn tu cac cay lai khac loai
E.alba
x E.urophylla va
E.tereticornis
x E.
saligna, trong dd cd hai ddng vd tinh ndi tieng nhat va dugc trdng rdng rai
nhat la: E.
PFl

Mau cay vo
trung
i
Tai sinh
choi in vitro
Vudn vat
lieu giong
Rung trong
dong vo
tinh
Nhan choi
va phat
trien choi
II
Cac bude I va
II
Cac
bude tir
2.1
Cac bude tir 1.1
Cac
bude
1.3
va
Cac bude
1.4
va
Ghi
chii:
: Xu ly tao vat lieu ban dau cho nhan gidng md - hom.

ciin
cay nguyen lieu giay Phu Ninh,
Trung tam khoa hgc san xuat lam nghiep Quang Ninh, Xi nghiep gidng
thanh phd Hd Chi Minh, Trudng Dai hgc Lam nghiep [2].
Trong khi nang suat rimg tu nhien
cila
ta chi dat 2 - 6
m^/ha/nam,
nang suat rung trdng quang canh dugc trdng bang cay hat ciing chi dat 5 - 7
mVha/nam, thi nang suat rung trdng
ciia
mot sd gidng Bach dan mdi chgn
tao hoac mdi nhap dugc trdng bang cay md va cay hom cd the dat
15
- 20
mVha/nam [2].
Nhan gidng bang nudi cay md te bao hay vi nhan gidng la ten ggi
chung cho cac phuong phap nudi cay in vitro cho cac bd phan nhd dugc
tach khdi cay (George,
1993)
dang dugc diing phd bien de nhan gidng thuc
vat, trong dd cd cay lam nghiep. Cac bd phan dugc diing de nudi cay cd the
la chdi dinh, chdi ben, chdi bat dinh, bao phan, phan hoa, phdi va cac bd
phan khac nhu vd cay, la non, than mam [7], [8].
Cung vdi nhung ket qua ve cai thien gidng, Vien nghien cuu cay
nguyen lieu gidy va Trung tam nghien cuu gidng cay rung da nghien
ciin
thanh cdng ky thuat nudi cay md va giam hom cho mot sd ddng Bach dan
va mot sd gidng cay rung khac [4].
Nghien cuu nudi cay md cho gidng lai giiia Bach dan lieu va Bach

PNl
4,
PN2), den nay cac cdng trinh do cdng bd ung dung
vao san xuat quy md
Idn
chua nhieu.
1.4.3. Tinh hinh nghien
ciru
d"
nude
ngoai.
Nhd ung dung ky thuat nudi cay md vao nhan gidng, ngudi ta da tao
ra hang loat cay con ddng nhat ve kieu gen vdi sd lugng
Idn
ma cac phuong
phap nhan gidng vd tinh thdng thudng khac khdng the cd dugc. Nudi cay
md cd uu the hon cac phuong phap nhan gidng vd tinh khac la cd ty le
nhan gidng cao, tdi hang trieu cay mdi nam. Ngoai ra cd the ap dung nhieu
bien phap xir ly len nudi cay in vitro hon la cac phuong phap khac. Cay
gidng tao ra bang phuong phap nudi cay in vitro
vira
khoe, tre sinh ly va cd
the sach virus ngay ca khi mau lay tir dinh sinh trudng
cila
nhiing cay da
nhiem virus
[12], [14].
Viec chgn vat lieu ban dau cho vi nhan gidng la rat quan trgng,
khdng nhiing quyet dinh su thanh cdng ban dau ma ca qua trinh nhan tiep
theo.

md ta phan ung phat sinh re in vitro
cila
Eucalyptus globulus Labill
ddi vdi Ca, N, P, Fe, Mn, Zn, B, Cu d cac ndng do khac nhau. Phan tich
nhirng sd lieu ve pham tram binh thanh re, sd re, chieu dai re va thdi gian ra
re.
Sd re va chieu dai re bi anh hudng dang ke bdi dinh duong khoang,
trong khi thdi gian ra re va phan tram hinh thanh re cd ve cd quan he mat
thiet vdi hieu luc
cila
auxin. Sd re bi anh hudng bdi ngudn Ca, N va Zn,
trong khi chieu dai re lai bi anh hudng bdi ndng do cua P, Fe, Mn va bdi
ngudn N. Du tren nhimg ket qua co ban dd, hg danh gia cac td hgp khac
nhau ciia cac chat khoang d vai ba ndng do ddi vdi mdi gian doan hinh
thanh re khac nhau
[15].
Sophie Nourissier and Olivier Monteuuis (2007) thuc hien mot loat
cac thi nghiem nudi cay tao re in vitro hai ddng Bach dan lai vd tinh: 147
va 149 {E. urophylla
XE.
grandis) tren mdi trudng MS cai tien. Vdi ty le ra
re la
81%)
ciia ddng 147, hg da khang dinh hieu qua dang ke
cila
5
pM
IBA
va
12.5 pM

tri
nhirng dac tinh tdt
cila
nd. Hai trong nhiing phuong phap nhan gidng
duy tri dugc nguyen ven nhiing tinh trang tdt tu ddi trudc cho ddi sau la
phuong phap nhan gidng bang nudi cay md va giam bom. Hai phuong phap
nhan gidng vd tinh tren cd y nghTa
Idn
trong viec khac phuc nhan td thdi
gian trong san xuat lam nghiep va tao ra cac cay gidng cho trdng rung ddng
vd tinh vdi nhieu uu viet: nang suat va do ddng deu
cila
san pham [7; 8;
11].
Viec nhan gidng cay rung bang phuong phap nudi cay md te bao va
giam hom de cung cap cay gidng tdt phuc vu trdng
rimg
dai tra dang la mot
hudng di dugc nhieu nude quan tam nghien cuu. Phuong phap chiet va
ghep cd nhugc diem bj ban che ve sd lugng nen khdng dap
ling
dugc cho
yeu cau san xuat
Idn
theo quy md cdng nghiep.
Vdi loai cay nguyen lieu giay ndi chung va tung ddng Bach dan ndi
rieng, mdi ddng hoac mot nhdm cac ddng cd the thich hgp vdi nhimg bien
phap nhan gidng khac nhau. De dua dugc mot ddng ra san xuat
Idn
theo

chgn mdi trudng nudi cay thich hgp trong nudi cay md la rat can
thiet. Vi mdi loai, ca the cay trdng khac nhau deu yeu cau mot ham lugng
dinh dudng khac nhau. Viec
lira
chgn mdi trudng dua vao cac tai lieu
nghien
ciin
cho ciing ddi tugng nudi cay hoac tham dd qua mot sd mdi
trudng dl xac dinh mdi trudng thich hgp cho mau nudi cay.
Sir dung chit dieu hoa sinh trudng trong mdi trudng nudi cay de tai
sinh mot each dinh hudng cac md nudi cay. Qua trinh nay dugc dieu khien
chil
ylu dua vao ty le
cila
cac hgp chat auxin, cytokynin ngoai sinh dua vao
mdi trudng nudi cay. Viec su dung chat dieu hoa sinh trudng dugc coi la
giai doan then chdt cua qua trinh. De tang he sd nhan, ta thudng dua them
vao mdi trudng dinh duong nhan tao cac chat dieu boa sinh trudng (Auxin,
11
Cytokynin, Gibberellin, ), cac chat bd sung khac nhu nude dua,
djch
chiet
nam men, ket hgp vdi cac yeu td nhiet do, anh sang thich hgp [8].
Khi dat dugc kich thude nhat dinh, cac chdi dugc chuyen tu mdi
trudng d giai doan nhan chdi sang mdi trudng tao re. Thudng 2-3 tuan, tu
nhirng chdi rieng le nay se xuat hien re va trd thanh cay hoan chinh. O giai
doan nay ngudi ta thudng bd sung vao mdi trudng nudi cay cac auxin la
nhdm bormon thuc vat quan trgng cd chiic nang tao re phu tir md nudi cay.
+ Ddi vdi giam bom:
Nhan gidng sinh dudng bang hom la bien phap dang dugc phat trien

ciru.
2.1.1.
Doi
tirgng
nghien cuu: mot sd ddng vd tinh Bach dan uu trdi,
nang suat cao da dugc cdng nhan gidng (giai doan 2000 - 2006):
PNl
16;
PN3d; PN54; PN46.
2.1.2.
Cham
sde vud'n
vat lieu: Theo quy trinh ky thuat nam 2002
ciia Vien NC cay nguyen lieu giay. Hang ngay tudi nude de dam bao
dii
do
am cho cay, thudng xuyen bdn phan, vun xdi, lam cd vudn cap hom.
Ddn cay tao chdi: dugc thuc hien dinh ky, bang each dung keo cat
ngang than
each
mat dat 20-30 cm. Thu chdi de giam hom sau 25 ngay.
2.1.3.
Nuoi cay mo:
Su dung phuong phap nudi cay tao chdi true tiep
tir
mau vat nudi cay
(doan chdi non), dugc bat dau bang tach dinh chdi hoac chdi nach tir cac
cay djnh nhan, sau dd khu trimg va dua vao nudi cay d mdi trudng phu
hgp.
Cac chdi dugc hinh thanh va dugc tach ra cay chuyen sang mdi trudng

+ Nhiet do: 25 - 29
°C.
Cac thi nghiem dugc bd
tri
hoan toan ngau nhien theo phuong phap
thue nghiem so sanh (tien hanh thi nghiem theo cac
Id
thi nghiem, so sanh
ket qua va ket luan) vdi 3 lan nhac lai, dugc quan sat theo djnh ky. Thuc
hien theo cac cdng thuc thi nghiem (TN):
Thi nghiem 1: Thu nghiem nudi cay nhan nhanh.
Vat lieu thi nghiem: chdi non cua 3 ddng vd tinh Bach dan da dugc
f
r
f
cdng nhan gidng cap qudc gia (PN3d, PNl 16, PN54, PN46).
Thi nghiem dugc tien hanh vdi mdi trudng MS cai tien dua tren nen
mdi trudng nudi cay MS (dugc ky hieu la
MSI).
Thanh phan mdi trudng:
Phu luc. Thi nghiem gdm cd 3 cdng thuc, mdi cdng thiic cd 3 lan lap lai,
cay 5 binh tam giac cd chua 50 ml mdi trudng nudi cay, mdi binh cay 6
mau.
Thi nghiem 2: Thu nghiem nudi cay phat trien chdi nham xac dinh
ddng cd kha nang nhan nhanh, 4 ddng vd tinh Bach dan dugc nudi cay tren
mdi trudng trong dieu kien nudi cay gidng nhau.
Vat lieu thi nghiem: chdi non ciia 4 ddng vd tinh Bach dan da dugc
cdng nhan giong cap qudc gia (PN3d, PNl 16, PN54, PN46).
Thi nghiem dugc tien hanh vdi mdi trudng dura tren nen mdi trudng
nudi cay MS cai tien - MS3 (dugc ky hieu la: MS3), mdi cdng thiic cay 6

IBA
1.0
mg/1.
- Chi tieu do dem:
+ So chdi/cum chdi.
+ Chieu cao chdi (cm).
+ So cap
la/cbdi.
+ Ty le chdi
hiin
hieu
(%>).
+ Ty le ra re (%).
2.1.4. Giam hom:
Dung phuong phap giam hom chdi, vat lieu giam hom la cac chdi
f
\
F
f
non, dugc lay tu chdi
ciia
gdc cay cap hom trong vudn vat lieu. Hom dugc
xii ly chat kich thich ra re trudc khi giam vao gia the. Phun suong
mil
dugc
thuc hien de cham sde bom giam hang ngay. Che do cham sde hom giam
tuy thugc vao thdi tiet bang ngay va timg giai doan.
Cac thi nghiem dugc bd tri hoan toan ngau nhien theo phuong phap
thuc nghiem so sanh (tien hanh thi nghiem theo cac
Id

- Dia diem trdng : Tam Thanh
(Phii
Thg), Lap Thach (VTnh Phiic).
- Cac chi tieu danh gia la:
+ Sinh trudng chieu cao: chieu cao
viit
nggn Hvn (m).
+ Sinh trudng dudng kinh: dudng kinh gdc Dg (cm).
+ Cap sinh trudng va theo ddi tinh hinh sau benh hai.
Chieu cao
viit
nggn (Hvn): do tu gdc sat mat dat tdi dinh nggn chinh.
Dudng kinh gdc (Dg): do tai vi tri sat mat dat.
- Dung cu do dudng kinh va chieu cao chuyen dung.
* Cap sinh trudng
ciia
than cay dugc phan 3 cap:
+ Cap I (ky hieu t): Cay sinh trudng tdt, sue sdng tdt, khdng sau
benh.
+ Cap II (ky hieu tb): Cay sinh trudng binh thudng.
f
r
+ Cap III (ky hieu x): Cay sinh trudng cham,
siic
sdng kem, bi sau
hoac benh lam anh hudng den
sue
sinh trudng.
- Tinh hinh sau, benh hai dugc danh gia theo hien tugng:
+ Cd bi sau benh hai (ky hieu c).

1.1.
Thiet bj, dung cu, nguyen vat
lieu
va hoa chat sir dung cho
nghien
ciru.
2.2.1.
Trang thiet bj va dung cu:
* Thilt bi:
til
cay vd tning, ndi hap, may do pH, can dien tii, dieu hoa
nhiet do, nhiet ke, am ke, den neon,
* Dung cu: panh, keo, dao cay, binh thuy tinh hinh tam giac 500ml,
binh thuy tinh hinh tru 250 ml, dTa, den cdn
2.2.2.
Hoa chat: (phu luc 2).
17
2.3.
Ket qua thuc nghiem va thao luan.
2.3.1.
Ddnh gid khd ndng nhdn nhanh (he so nhdn in vitro cua cdc
dong) vd khd ndng sinh trw&ng, phdt trien choi ciia cdc dong nghien
cwu.
Sau 4 tuan nudi cay, trang thai sinh trudng phat trien chdi Bach dan
in vitro dugc the hien ve chieu cao trung binh chdi, sd la trung binh/chdi,
sd chdi trung binh/cay (bang 01).
Bdng 01. Khd ndng sinh trudng phdt triin chdi Bgch ddn in vitro
ciia
cdc ddng nghien cim (sau 4 tuan nuoi cdy)
TT

ddng tu 2,38 cm den 3,37 cm).
+ Chieu cao TB chdi tir 1,6 - < 2,5 cm: ddng PNl
16
(chieu cao chdi dao
ddng tir 2,44 cm den 2,68 cm).
+ Chieu cao TB chdi < 1,6 cm: ddng PN46 (chieu cao chdi dao dgng tu
1,36 cm den 1,59 cm).
Sau 4 tuan nudi cay, sd cap
la/chdi
ciia cac ddng dugc chia thanh cac
nhdm nhu sau:
+ Chdi cd > 4 cap la/chdi: ddng
PN54
+ Choi cd cd tir 3 - 4 cap
la/choi:
ddng PNl
16,
PN3d.
+ Chdi ed < 3 cap la/chdi: ddng PN46.
18
Sir
phat sinh chdi tren cay cua cac ddng ndi tren dugc sap xep theo
cac nhdm nhu sau:
+ Cum choi cd TB >4 chdi: ddng PNl 16, PN3d.
+ Cum chdi cd TB
tir
3- <4 choi: ddng PN54.
+ Cum chdi cd TB tir 1- <3 choi: ddng PN46.
He sd nhan chdi Bach dan in vitro la chi tieu danh gia kha nang nhan
'

2
3
4
Ddng
PN54
PNl
16
PN3d
PN46
Ty le choi huu hieu (%)
Loai 1
(tot)
57,26
55,36
53,70
5,66
Loai 2
(TB)
29,82
13,99
26,31
30,33
Loai 3 (xau)
12,92
30,65
19,99
64,01
Trang thai
chdi
+++