BỘ GIÁO DỤC VÀ ĐÀO TẠO BỘ NÔNG NGHIỆP VÀ PTNT
VIỆN KHOA HỌC NÔNG NGHIỆP VIỆT NAM NGUYỄN THỊ BÍCH THÙY

NGHIÊN CỨU ĐẶC ĐIỂM SINH HỌC VÀ CÔNG
NGHỆ NHÂN GIỐNG, NUÔI TRỒNG NẤM SÒ VUA
(PLEUROTUS ERYNGII) VÀ NẤM VÂN CHI
(TRAMETES VERSICOLOR) Ở VIỆT NAM LUẬN ÁN TIẾN SĨ NÔNG NGHIỆP
Hà Nội - 2014i
LỜI CAM ĐOAN






5 4 TÁC GIẢ
Nguyễn Thị Bích Thùy

Nguyễn Thị Bích Thùy
              


 


 xuyên 
G, 
./.
4
Tác giả
Nguyễn Thị Bích Thùy iii
MỤC LỤC

 i
L ii
M iii
Danh  vi
D  vii
Danh  ix
 1
  5
1.1 5
 5

chi  47

 51
 52
 55
 56
 57
:  58
3.1. N 58
3.1.1. 
 58
3.1.2.  
 64
3.1.3.  69
3.1.4. 
 71
3.1.5. 
 74
 78 v

Vân chi 80
3.2.1.  80
Vân chi 85
 86
3.3.1.  . 87

 106

CTAB
Cetylmethyl Ammonium Bromide


dNTPs
Deoxyribonucleotide Triphotphates
EDTA
Ethylene Diamine Tetraacetic Acid
g
Gram
KLC

PCR
n gen)
PGA
Potato glucose agar
ppm
Parts per million
PSK
polysaccharide krestin
PSP
polysaccharide peptide
RAPD
Random Amplified Polymorphism DNA
SK

T

Taq Polymerase
Thermus Aquation Polymerase

3.7. 
 82
3.8.  83
3.9. 
 88
3.10. 
 91
3.11. 
 94
3.12. 
  98 viii
3.13. 
 100
3.14. 
 103
3.15. 
 107
3.16. 
 109
3.17. 
 112
3.18. nh h vua .115
3.19.            
 117
3.20. 
Sò vua 119
3.21. 

0
C 61
3.4. 
4

0
C 61
3.5. 
6
18 ± 1
0
C 61
3.6. 
1

0
C 61
3.7. 
2

0
C 61
3.8. 
5

0
C 61
3.9. 
6
22 ± 1

0

0
C 62
3.16. 
6
sau kh
0
i 18 ± 1
0
C 62
3.17. -
1
62
3.18.   vua 66
3.19. 
1
68
3.20. 
2
68 x
3.21. 
3
68
3.22. 
4
68


 70
3.32. 
khác nhau 73
3.33.  Tra-1
0
C 74
3.34. Tra-1 1
0
C 74
3.35. HTra-2 1
0
C 74
3.36.  77
3.37.  77
3.38. - 79
3.39. - 79 xi
3.40. - 79
3.41. 
lux - bên trái n 79
3.42. - 79
3.43. - 79
3.44. - 79
3.45. -2)  79
3.46. -
 81
3.47. - 81

3.65. - 123

29,30,60,61,62,66,68,69,70,73,74,77,79,81,84,85,96,101,104,105,110,111,123

1-28,31-59,63-65,67,71,72,75,76,78,80,82,83,86-95,97-100,102,103,106-109,112-
122,124- 1
MỞ ĐẦU

1. Tính cấp thiết của đề tài
-   và
g tham gia vào   
T nông nglâm
lõi ngô, thân ngô Vng

  - lâm nghi góp

Ngày nay, 
c
 các
- 

 
(Pleurotus eryngii (DC.) Quél) gon, 
 

cao,
, 

cao, i trung
10 - 25 ngày 
trong vòng 2 tháng

xu

3 - 5 

p. 3
           
    
. 
 
 
nêu trên,    - 
nói c    - 
  Nghiên cứu đặc điểm sinh học và công nghệ
nhân giống, nuôi trồng nấm Sò vua (Pleurotus eryngii) và nấm Vân chi
(Trametes versicolor) ở Việt Nam”.
2. Mục tiêu của đề tài


 


3. Ý nghĩa khoa học và thực tiễn
3.1. Ý nghĩa khoa học 5
CHƢƠNG 1
TỔNG QUAN TÀI LIỆU

1.1. Tình hình nghiên cứu, sản xuất nấm trên thế giới và Việt Nam
1.1.1. Tình hình nghiên cứu và sản xuất nấm trên thế giới
1.1 

n nay,         sinh  
             

 
  
loài câ

             
- 
      


       
             
        
   



mannitol SK  3 lactose cho SK 
1,47g/lít (Sukumar và .
Khi  
            
n
8,6 g/1000m 
ngày               7
,    pH  môi
nuôi  2,92;   
lên 4,39 (Bolla , 2010).
Adebayo          

MgSO
4
.7H
2
O (0,2g); K
2
HPO
4
(1,0g); NH
4
SO
4
(5,0g); glucose (9,75g); CNM
             
 ar  lactose, glucose, sucrose, mannitol, maltose, galactose,

s             
c pH
(Bolla
.
axit amin 
  axit amin 
axit amin 
Adebayovà Ugwu, 2011).
(T. versicolor)  
 
 , 2005).
Kong         
10
o
C; 15
o
C; 20
o
C; 25
o
C; 30
o
C; 35
o
C, 

o
-15
o


mt s kt lun v u kin thích hp cho nm Mng tt trong
 ng l  n cacbon là 1,6% glucose; ngu   
ng có giá tr pH = 6,5; nhi nuôi là 25ºC.
Liu và Cng s (2010) nghiên cng dch th thích hp 
nuôi ging nm Kim châm LP03,  t lung dch th
nuôi cy tt nht gm: pepton; glucose; bt ngô; MgSO
4
.7H
2
O; KH
2
PO
4
;
vitamin B
1
, u kin nhi 25±3ºC và ch  lc 160 vòng/phút phù
hp cho s phát trin si nm, c ging nm phát trin tc
KLC u.
Marquez-Rocha và C (1999)  
 trong biorea 
và c


 
. 10


 thì 
tán (Gibbs và .

 
  
              
 ít 
 và Lounghlin, 2000).
         











              
.
 (P.eryngii) bao  
 và  sinh 
  hình thành các  phân nhánh 
   trên . Sau khi 
kích thích    