59

|P12 |TGCTTGAATTTGTCATGTCCAAGG | 53 | 59 | -49 | 42 | 26-49 |
|M4 |CCTGCAATAGAGAGGAATAATCGC | | 56 | -50 | 46 | 923-946 |
| | | | | | | |
|P14 |GCAGATGCTTGAATTTGTCATGTCC | 53 | 59 | -50 | 44 | 21-45 |
|M6 |GCAATAGAGAGGAATAATCGCTCC | | 55 | -49 | 46 | 920-943 |
| | | | | | | |
|P13 |ATGCTTGAATTTGTCATGTCCAAGG | 53 | 59 | -50 | 40 | 25-49 |
|M6 |GCAATAGAGAGGAATAATCGCTCC | | 55 | -49 | 46 | 920-943 |
| | | | | | | |
|P12 |TGCTTGAATTTGTCATGTCCAAGG | 53 | 59 | -49 | 42 | 26-49 |
|M6 |GCAATAGAGAGGAATAATCGCTCC | | 55 | -49 | 46 | 920-943 |
| | | | | | | |
|P32 |CAGAGTGATCCATCGGAAGC | 52 | 53 | -43 | 55 | 196-215 |
|M9 |CCTGCAATAGAGAGGAATAATCG | | 53 | -46 | 43 | 924-946 |
| | | | | | | |
|P32 |CAGAGTGATCCATCGGAAGC | 52 | 53 | -43 | 55 | 196-215 |
|M8 |CTGCAATAGAGAGGAATAATCGC | | 53 | -46 | 43 | 923-945 |
| | | | | | | |
|P36 |CATGTAGCGCAGAGTGATCC | 52 | 51 | -42 | 55 | 187-206 |
|M12 |TGCAATAGAGAGGAATAATCGC | | 52 | -45 | 41 | 923-944 |
| | | | | | | |
|P32 |CAGAGTGATCCATCGGAAGC | 52 | 53 | -43 | 55 | 196-215 |
|M12 |TGCAATAGAGAGGAATAATCGC | | 52 | -45 | 41 | 923-944 |
| | | | | | | |
|P32 |CAGAGTGATCCATCGGAAGC | 52 | 53 | -43 | 55 | 196-215 |
|M11 |CAATAGAGAGGAATAATCGCTCC | | 52 | -46 | 43 | 920-942 |
| | | | | | | |
|P28 |AGAGTGATCCATCGGAAGCC | 52 | 54 | -44 | 55 | 197-216 |
|M9 |CCTGCAATAGAGAGGAATAATCG | | 53 | -46 | 43 | 924-946 |

 Vùng bắt cặp: 197-216 và 920-943
 Trình tự primer 5’ – 3’
P28 5’-AGAGTGATCCATCGGAAGCC-3’
M6 5’-GCAATAGAGAGGAATAATCGCTCC-3’

60

Phần V
Kết luận và đề nghị
5.1 Kết luận
Qua điều tra và chẩn đoán, chúng tôi thu nhận đƣợc các kết quả nhƣ sau:
 Tỷ lệ nhiễm TSWV theo địa bàn điều tra
- Xã Nhuận Đức: 12,1%
- Xã Phƣớc Thạnh: 12,5%
- Xã Lộc Hƣng: 0%
 Tỷ lệ nhiễm TSWV theo triệu chứng
- Lá khảm vàng xanh, cong: 37,5%
- Lá chấm vàng xanh, nhăn nheo, co lại, dày, giòn: 57%
- Lá khảm nặng có chấm đen, lủng lỗ:53%
- Khảm vàng nhạt trên cả rìa lá, lá nhỏ: 25%
 Tỷ lệ nhiễm trên trái
- Trái nhiễm: 33%
- Không nhiễm: 67%
Nhƣ vậy khi điều tra trên đồng ruộng thì chúng ta có thể đánh giá bệnh TSWV dựa
trên các triệu chứng trên. Đánh giá bệnh dựa vào giống và tuổi cây thì chƣa thực hiện
đƣợc vì bà con nông dân chƣa thực sự quan tâm đến giống mà họ đang trồng, và thời
gian nghiên cứu thì lại rơi vào cuối vụ, tất cả đều cho trái nhƣ nhau.
5.2 Đề nghị
 Tiếp tục nghiên cứu mức độ gây hại của TSWV theo giống và độ tuổi.
 Nghiên cứu khả năng lây lan của TSWV trên các đối tƣợng khác.

ớt (Capsicumannum L.). Bước đầu xây dựng qui trình phòng trừ tổng hợp bệnh
thán thư ớt.
7. Nguyễn Văn Tuất, 2002, Kỹ thuật chẩn đoán và giám định bệnh hại cây trồng. Nhà
xuất bản Nông nghiệp, Hà nội, trang 69.
8. Bùi Cách Tuyến, 1998, Bản dịch tiếng việt, Tài liệu hướng dẫn đồng ruộng, Bệnh
hại cây ớt. Nhà xuất bản Nông nghiệp, trang 42 – 56.
9. Bùi Trang Việt, 2002, Sinh học phân tử, ĐH Quốc gia TP. HCM Khoa Sinh học -
Trƣờng ĐHKHTN.

TIẾNG NƢỚC NGOÀI
10. Alfred Nobel Dr. Hercules, CA 94547 USA, Inc, 2000, Aurum Total RNA Mini
Kit Instruction Manual Bio-Rad Laboratories, trang 26-27.
62

11. Biowave vol.6 No.23 2004, tomato spotted wilt virus.
<www.bric.postech.ac.kr/webzine/content/review/virology/2005/v7n12/pv0616.pdf >
12. Chemicon International, Inc - 28820 Single Oak Drive - Temecula, CA 92590,
Introduction to Antibodies - Enzyme-Linked Immunosorbent Assay (ELISA)
<
13. G.A. Dafalla, Plant Pathology Centre, Faculty of Agricultural Sciences,University
of Gezira, Wad Medani, Sudan.
<www.iita.org/info/virology/pdf_files/18-24.pdf>
14. Introduction to Microbiology: Virus Genomes, 2005.
<
15. J.Morris, Central Science Laboratory, Sand Hutton, York, Y041 1LZ, UK.
<www.csl.gov.uk/science/organ/ph/diagpro/tospovirus.pdf>
16. John R. Crowther, 2001, The Elisa Guidebook – Volume 149, trang 12 – 34.
17. Ken Pernezny-Everglades research and Education Center University of Florida,
Belle Glade; Pamela D. Roberts-Southwest Florida Research and Education Center
University of Florida, Immokalee; John F. Murphy-Auburn University, Auburn,

<
26. Vernon E Coyne, M Diane James, Sharon J Reid and Edward P Rybicki,Molecular
Biology Techniques Manual, Third Edition, 1996.
<
27. <www.apsnet.org/pd/pdfs/2002/0926-01R.pdf>.
28. <www.promega.com/guides/rna_guide/amplifyingrna.pdf>.

PHẦN VII
PHỤ LỤC

Phụ lục 1: Trình tự Nucleotide của genome TSWV
1 agagcaattg tgtcagaatt ttgttcataa tcaaacctca cttagaaaat cacaatactg
61 taataagaac acagtaccaa taaccataat gtcttcaagt gtttatgagt cgatcattca
121 gacaagagct tcagtctggg gatcaactgc atctggtaaa gctgttgtag attcttactg
181 gattcatgaa cttggtactg gttctcaact agttcagacc cagctgtatt ctgattcaag
241 aagcaaagta gtcctttggc tatactgcaa agtagggatc ttccctgtga agaagaagag
301 atttctttct cagcatgtgt atatccctat ttttgatgat attgatttta gcatcaatat
361 tgataactct gttctggcac tatctgtttg ctcaaataca gtcaatgcta acggagtgaa

2221 tgggtcgatc ccgaggtcct tgtattttgc atcctgatat atagccaaga caacactgat
2281 catctcaaag ctatcaactg aagcaataag aggtaagcta cctcccagca ttatggcaag
2341 tctcacagac tttgcatcat cgagaggtaa tccataggct tgaatcaaag gatgggaagc
2401 aatcttagat ttgatagtat tgagattctc agaattccca gtttcttcaa caagcctgac
2461 cctgatcaag ctatcaagcc ttctgaaggt catgtcagtg cctccaatcc tgtctgaagt
2521 tttctttatg gtaattttac caaaagtaaa atcgctttgc ttaataacct tcattatgct
2581 ctgacgattc tttaggaatg tcagacatga aataacgctc atcttcttga tctggtcgat
2641 gttttccaga caaaaagtct tgaagttgaa tgctaccaga ttctgatctt cctcaaactc
2701 aaggtctttg ccttgtgtca acaaagcaac aatgctttcc ttagtgagct taaccttaga
2761 catgatgatc gtaaaagttg ttatatgctt tgaccgtatg taactcaagg tgcgaaagtg
2821 caactctgta tcccgcagtc gtttcttagg ttcttaatgt gatgatttgt aagactgagt
2881 gttaaggtat gaacacaaaa ttgacacgat tgctct
Hình pl.1a: Trình tự Nucleotide genome S (Short)

1 agagcaatca gtgcatcaga aatataccta ttatacattt tgctaagaat caatcaacta
61 cattacacaa gctcctctac cttaggctgt tgaactcgaa atgttgactc ttttcggtaa
121 caagaggcct tctaagtctg taatggcagt gttgaagtct caaaaccatg gtcttcttct
181 gatgaaaagc ttgctttaac ccggaaagga tgaaggtcct ttagtttcac ttgctaaaca
241 caaagccatg gatgcatcca aaggaaagat actgttaaac attgagggaa catcttcctt
301 tggaacctat gaatctgatt ccatcacaga gtcagaaggt tatgatcttt ctgctagaat
361 gatagtagat acaaaccatc atatctcaaa ctggaaaaat gatctttttg ttggcaacgg
421 aaagcaaaat gctaataagg ttatcaagat ctgtccgact tgggacagca gaaaacaata
481 catgatgatt tccagaattg tgatatgggt ctgccccact ataccaaacc ctacaggaaa
541 acttgtggtt gctttaattg atcccaacat gccatctgga aagcaagtca tcctgaaggg
601 tcaggggaca ataactgatc ctatctgctt tgttttttat ctgaactggt ctattccgaa
661 gatgaacaac accccagaaa actgttgtca gctgcatttg atgtgtagcc aagaatacaa
721 gaaaggggtt tcttttggta gtgtcatgta ttcttggaca aaagagtttg gtgattcacc

2581 tccccaataa gaagttggtg ttacacaaaa atcttggaaa cctgttaaag cttgattttt
2641 cctgcaagtg tcgcagtttc ctgtacaagt ggaataaaaa tccgtgtggg tgctttggat
2701 gggagctgtc gtatattttt ctgacacttc ataatgaatt cccacacttt tgatataaat
2761 cacaaatttt ttggctgttt ctgaggtctt gtcatttagc atgaatatag ttcctcctcc
2821 tcctaaaaga gattgttcta tcatatatct atatttcccg tctacaacag aatcaaagat
2881 taatgattgc ctgggcagaa tgttctctgg tatgcttgtt tcgtttttgt ttaaggagtc
2941 tccggcaacc attccagaaa ttttgccctg acggttatag gaatctatta taccatttct
3001 caatctctta gcctcataca gaccaactga tgttaaccct aaagagcttc ctgtataatc
3061 cgaaaaccca ttgtacaaag gtttgtttcg gaataaatct aggtattcgc aacctaatct
3121 gcattttata agattatcaa tagatgggcg tgcaatgcaa ttctggttct ctatgcaatc
3181 attaggacct tctataacaa tattagtgcc aaagatactc tctatgatct tatcttcttt
3241 tacattgcag taacattgat ctttttcaga gcatttttca aatttgcttg taaccaaaaa
3301 tggacagcct ggaacataaa agcatccact caaacattgg gttgtttgag ccatagacat
3361 gggcatctga gacaaaatga ctaaacctat caaaatttcg gtcacaaatt ttagcaaact
3421 caagctcagc ttagtgttca ctattagatg gaaccattcc atgctagtcc acttatgttt
3481 gttgtagtca tgatctgctt ctttggatag tatgggacac tctgaagaat gctcttttga
3541 agctttgctt ttgttgcaaa tgcagacttt agtacactca tgtgtgacta tgcacagatt
3601 gccgcagtta gaacatttta atgggaaata tttccataag caatttatga gcaataagac 3661 agggtatgta atcaagccca taagatcata ccagagaaag agaggtttag tcgtcttgtt
3721 cactaaccat cggataggga aatagatcaa caaagctatc aatatcaacc ttatccaaga
3781 aaaattgatg caggctgttt gcttataaat actttttgaa tatttgatta tgcaatctct
3841 gactcttttg tttgtttttg gtattttggc tgatttgtca ccacacaaga gattgtgttc
3901 accatccaac atttcctcag taaaagtgat acttgctgat ccagaaaaag atataacctt
3961 gtgttccaca ttttcaccag gtttttttat caaataaccc atgatcttct cagggctagt
4021 gatgcttaca gtatagggat ttgcgaagtt tgatttagtt attttgcagt caccggataa
4081 ctttacagtt tgtaatgata ctgttccatt agtggggtat gagttgtaag ttataggata
4141 attatcttgt gtcaggcttt ctgaaatgaa gaattttcct cctactgaaa agtgtctttt
4201 cttgtctagc ttggtaatgg gaataaccgg gactttggag aatctctttg gcaaatttaa

1021 cctactgaga tagcctattc cgaagatatt gaaagaataa ttgattcact tgttacagat
1081 gaaatcccta gagaggaaat aatacatttt ttgtttggaa atttctgttt ccacattgaa
1141 acaatgaatg accagcatat agctgacaaa tttaaagggt accaaaactc ttgtatcaat
1201 ttaaaaatag agccaaaagc tgatttagct gatttgaaag accacttaat ccaaaagcag
1261 caaatatggg aatctctgta tggaaaacac cttgagaaga tcatgcttag aattagagaa
1321 aaaaagagaa aagaaaaaga gatacctgac ataaccacag cttttaacca gaatgctgct
1381 gaatatgaag aaaggtatcc taactgtttc aatgatctct ctgaactaaa actaactttc
1441 catgacttgg tccccagttt gaagatagaa ttgagctcag aggtagatta caacaacgca
1501 attattaaca agtttcggga gagcttcaaa agttcttcaa gggttattta taatagccca
1561 tatagtagca taaataacca aacaaataaa gcaagagata taacaaactt agttagactg
1621 tgtttagcag agctaagttg tgatacaacg aaaatggaaa agcaggaact tgaagatgaa
1681 atagatataa acaccgggag tatcaaagtt gagagaacaa aaaagtctaa agaatggaat
1741 aagcaaggtt cgtgtttaac cagaaacaaa aatgaatttt gcatgaaaga tacaggcagg
1801 gagaacaaaa ctacctattt taaaggctta gcagtaatga atataggaat gagttctaag
1861 aaaagaattc taaaaaaaga agaaataaaa gaaaggatct ctaaaggctt ggaatatgat
1921 acctctgaaa ggcaggctga cccaaatgat gattactcaa gtatagacat gtcttctctg
1981 actcatatga aaaaactgat aaggcatgac aatgatgata gcttaagtgg taaaagattt
2041 aagggctctt tttttctact tcataatttt aatataatag aggatggtaa gatcacatct
2101 gttttcaata attatgctaa aaatcctgaa tgcttgtaca ttcaagattc agtactgaag
2161 actgaattag agacttgcaa aaagataaac aaattatgca atgatctagc catttaccat
2221 tactctgaag acatgatgca attctccaaa ggtttaatgg tggctgacag gtacatgact 2281 aaagaaagtt tcaagatatt aaccacagca aatactagca tgatgctatt agcattcaaa
2341 ggggatggaa tgaacaccgg aggatcggga gttccttaca tagcattgca tatagtggat
2401 gaagacatgt cagatcattt taacatatgt tatactaaag aaatttatag ctatttccga
2461 agtggtagta attacattta tataatgagg ccgcagagac taaaccaggt gaggctgctg
2521 aggcttttca aaacgcctag taaagttcct gtatgttttc cacaattttc aaagaaagct
2581 aatgaaatcg gaaaatcgct gaaaaataaa gatatagaaa aagtaaatct cttttctatg
2641 acaatgactg taaaacagat attaataaat attgtgtttt catctgtcat gataggaact

4501 tctgatgata atgcaacatc attaatagcc agtggagagg ttgataaaat gctgacagac
4561 ttttcaagct catctctgcc agaaatgttg tttagaagca ttgaagctca tttcaaaagt
4621 ttttgcataa ctttgaaccc aaaaaagagt tatgcttctt catcagaagt agagtttata
4681 tctgaaagaa ttagtaaatg gagcgattat tcctctctat tgcaggcatt tagcaaactg
4741 ttgcacagaa tcttcgcata taagttattt gatgatctaa tgtcactcag tatacatgtt
4801 acaatgcttc tgagaaaagg ctgtcctaat gaagttatac cttttgctta tggggctgtg
4861 caggtacaag cgttaagcat ctattcaatg cttcctggtg aagtgaatga tagtattaga
4921 atttttaaca agcttggagt aagtttaaag tcaaacgaga ttcccacaaa catggggggc
4981 tggttgacct ctcctataga gccgttgtct atattaggtc catcatcaaa tgatcaaatc
5041 atctattaca atgtgataag agattttttg aacaaaaaaa gtttagaaga agtaaaagat
5101 agtgtctctt cttccagtta tctacagatg agattcagag agctaaaaga aaagtatgaa
5161 agaggaactc tggaagaaaa agataaaaag atgatatttc ttatcaatct gtttgagaaa
5221 gcatcagtgt ctgaagattc agacgttcta acaattggga tgaaatttca aactatgtta
5281 actcagatta taaaattacc taattttata aatgagaatg ctttaaacaa gatgtcaagt
5341 tataaagatt tttcaaaact ttatcctaat ttaaaaaaga atgaagattt atataaaagc
5401 actaagaact taaagataga cgaggatgct gttttagagg aagatgagtt atataagaag
5461 attgcatcta gcttagaaat ggaatctgtc catgacataa tgataaaaaa tcctgaaaca
5521 attctgatag caccattgaa tgatagagat tttttactta gtcagctgtt catgtacaca
5581 agcccttcta aaagaaacca gttatcgaac caatctacag agaaacttgc tttagataga
5641 gtgttaaggt caaaagctag aacatttgta aacatttctt ccactgtgaa gatgacttat
5701 gaagaaaaca tggaaaagaa aatcttagaa atgctaaaat ttgatttaga ttcatattgt
5761 tcatttaaaa catgtgtaaa tctagttatc aaggatgtta atttcagcat gctgattcca
5821 atattagatt ctgcataccc ttgtgaatct aggaaaagag ataactacaa tttcaggtgg
5881 tttcagactg agagatggat acctgttgtt gaaggctctc cgggactagt agtaatgcat
5941 gctgtctatg gatcaaatta tatagagaac ttaggtttaa aaaacatccc tctaacagac
6001 gatagtatta atgttttaac aagcacgttt ggaacaggtt taatcatgga agatgtaaaa 6061 tccctagtta atggcaaaga cagcttcgaa acagaggctt ttagcaattc taatgaatgt
6121 caaagattgg tgaaagcatg caattatatg atagcagcac aaaacaggct tttagcaatt

7981 gtgttgaggc tagatgagga agaaatgtat gaaggtttga tcaaagaaat gaaaattaaa
8041 aggaaaaaga aagggttttt atttccagca aacacacttc tactaagtga gttgataaag
8101 ttcttgattg gaggaataaa gggaaccagc tttgatatag agacattgtt acggaacagt
8161 tttagaccag acatattttc aactgacaga ttgggaagat taagttccag tgtacctgca
8221 ctcaaagttt atgcaactgt ttatatggaa tataagaatg tcaattgtcc tttaaatgag
8281 atagctgaca gcttagaagg ttatctaaaa ctgacaaaaa gcaggtccaa ggaacatttc
8341 ttgtctggaa gagttaaaaa agctttgata caattaagag atgaacaatc gcgaactaaa
8401 aaactagagg tctataagga tatcgcaaat ttccttgcta ggcacccact atgtttatca
8461 gaaaaaacat tgtatggaag atatacctac tctgatatca atgattatat catgcaaaca
8521 agagagatta ttttgagtaa aataagtgag ttggacgagg ttgttgaaac agatgaagac
8581 aatttcttgc ttagttatct aagaggggaa gaagatgcct ttgatgaaga tgagcttgat
8641 gaagaagaag acacagatta aattgaaagt aatgactaac aatccatgaa taacagatta
8701 gatataactt agaatataaa tttattgcta ttttagaatt agattagatc tacttagcct
8761 aaaacaattt ggtgaaccaa atctatagtg tatataaatg tagagtcccg gtatagtttc
8821 actggaggga attcttatgt aatttgtaaa gtctggctgt ggagaggtta tatgttttag
8881 ttgtacctga ttgctct
Hình pl.1c: Trình tự Nucleotide genome L (Long)