BỘ GIÁO DỤC VÀ ĐÀO TẠO
TRƢỜNG ĐẠI HỌC NÔNG LÂM THÀNH PHỐ HỒ CHÍ MINH
BỘ MÔN CÔNG NGHỆ SINH HỌC
 KHÓA LUẬN TỐT NGHIỆP

PHÂN LẬP VÀ ĐỊNH DANH MỘT SỐ VI SINH VẬT CÓ
KHẢ NĂNG KÍCH THÍCH SINH TRƢỞNG THỰC
VẬT Ở VƢỜN QUỐC GIA CÁT TIÊN

Ngành học: CÔNG NGHỆ SINH HỌC
Niên khóa : 2003-2007
Sinh viên thực hiện: PHAN TRUNG HẬU


PGS.TS. BI VĂN LỆ PHAN TRUNG HẬU
ThS. KIỀU PHƢƠNG NAM

T
9/2007

iii
LƠ
̀
I CA
̉
M ƠN


, . 
















.
 














 










.
, 

 , 

 , , tin





9/2007


iv


Phân lập và định danh một số vi sinh vật có khả năng kích thích
sinh trƣởng thực vật ở vƣờn quốc gia Cát Tiên”      


Kết quả:


 .............................................................................................................. iii
t .................................................................................................................. iv
 ................................................................................................................... v






 ..................................................................................... viii






 ................................................................................................. ix
Danh  .............................................................................. xi






 ................................................................................................ xii

Chƣơng 1: MỞ ĐẦU ............................................... Error! Bookmark not defined.
1.1.  .......................................................... Error! Bookmark not defined.
1.2.  ............................................................. Error! Bookmark not defined.

) . Error! Bookmark not
defined.
3.2.3.3.  Error! Bookmark not defined.
3.2.3.4.  .................................... Error! Bookmark not defined.
3.3.  .................................... Error! Bookmark not defined.
3.3.1. n ..................................... Error! Bookmark not defined.
3.3.1.1.  .......................... Error! Bookmark not defined.
3.3.1.2.  ......................................... Error! Bookmark not defined.
3.3.1.3. 
... Error! Bookmark
not defined.
3.3.2.  .......................................................... Error! Bookmark not defined.
3.3.2.1. in
vitro in vivo ........................................................... Error! Bookmark not defined.
3.3.2.2.  .............................................. Error! Bookmark not defined.
3.3.2.3.  ......................................... Error! Bookmark not defined.
3.3.3.  ......................................... Error! Bookmark not defined.
3.3.3.1.  .... Error! Bookmark not
defined.
3.3.3.2.   ........................................... Error! Bookmark not defined.
3.3.3.3.  ........................................ Error! Bookmark not defined.

viii
3.3.3.4.  ................. Error!
Bookmark not defined.
3.3.3.5.  ....................... Error! Bookmark not defined.
3.3.4.  ........................................ Error! Bookmark not defined.
3.3.4.1.  ... Error! Bookmark not defined.
3.3.4.2.  ....... Error! Bookmark not
defined.


Bp Base pair
EDTA Ethylene-Diamine-Tetraacetic-Acid
MS 
CMS 2% casein
MMS 
Gram (-) 
Gram (+) 
PPFM Pink-Pigment Facultative Methylotrophic
PCR Polymerase chain reaction
MSo 
DNA Deoxyribonucleotide Acid
Taq Tag polymerase
LDC Lysine decarboxylase
PHB Poly-beta-hydroxybutyrate

ix
DANH MỤC CÁC HÌNH

TRANG
 (
 Methylobacterium
 .......................................................................... 6
 ................................................. 10
Azospirillum brasilense  ............................................... 11
 .................................................... 41

 ............................................................................................. 49

 .............................................................................................................. 49


TRANG
  ............................................................................ 24

 ................................... 47

  .......................... 48

 So ..................................................... 51

So .............................................. 51

610nm

 .......................................................................................................... 61
 ........... 61

 ..................... 62
 
 .................................... 62

xii
DANH MỤC CÁC BẢNG

TRANG
 
14GNR ............................................................................................... 37

  MS (Murashine & Skoog, 1962) ............... 47




t       


 
          
  












 2

Phân lập và định danh một số vi sinh vật có khả năng kích thích sinh
trƣởng thực vật ở vƣờn Quốc gia Cát Tiên

1.2 Mục tiêu
 

Chƣơng 2
TỔNG QUAN TÀI LIỆU
2
2.1 Vƣờn Quốc gia Cát Tiên [23]
  

              

 VQGCT
               

Nam.
 Hệ thực vật
 
 Dipterocarpaceae
Lagertroemia sp.
Saccharum
sp. Hydrocarpus sp. Ficus benjamina 

1
 
to
 Hệ động vật 4
 


 Crocodylus siamensis           

 10
10
  [18].
+ Eukaryote            
   vi

6
-10
9

0
7
, 10
5
-10
6

3

2
-10
3

c

8
-10
9
 [6].
  

methyl
(B)  [20]

Các vi sinh vật nội sinh (endophytic)
 (endophytic)  




 vsv             
            
  [14]. 7
2.2.1 Phân nhóm vi sinh vật tƣơng tác với thực vật dựa vào bản chất của sự
tƣơng tác [16]

 

 




- 
- 
- 
-   
2.2.1.1 Các vi sinh vật ức chế tác hại của mầm bệnh lên thực vật: 













 :
-1,3-glucanase...




 Pichia anomala, P. membranifaciens,R. glutinis, C.
laurentii, A. pullulans, Tilletiopsis albescens, -1,3-

















phenylalanine ammonia lyase phytoalexin, 

 






.
2.2.1.2 Vi sinh vật gia tăng dinh dƣỡng cho cây [16]


  

 


 
               
 
 10
Sự gia
tăng độ
mật
thiết của
các
tƣơng
tác
1. Vi sinh vật cộng sinh cƣ trú ở vùng gian bào
(Endosymbionts)
Ví dụ: 
2. Vi sinh vật cƣ trú ở vùng ngoại bào
(Ectosymbionts)
Ví dụ: t

3. Vi sinh vật bên trong mô cây chủ (Endophytes)
Ví dụ: Herbaspirillum sp. 
4. Vi sinh vật chỉ sống trên bề mặt cây
Ví dụ: Azospirillum  Klebsiella
Caesalpinoideae

 

Hình 2.2: 
 [16].
11
 do. 
nitr

Azospirillum 
 
Gluconacetobacter
diazotrophicus Herbaspirillum Azospirillum sp. 
  B 
ate 
 tan
-ketogluconic acid,
             
               

3+
, 

2+
) [16].