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PHỤ LỤC

HÓA CHẤT CHO ĐIỆN DI
 TBE (0,5X)
Tris HCl 3,94 mg
Acid Boric 1,39 mg
Na
2
EDTA 93,06 mg
Nước cất vừa đủ 500 ml
pH = 8,3
 Agarose (1,65%)
 Loading dye
Bromophenol blue 0,25%
Sucrose 40%
TE 1X vừa đủ 100%

HÓA CHẤT CHO NHUỘM GEL
TBE 1X
Ethidium bromide 10 mg/ml

MÔI TRƯỜNG VÀ THUỐC THỬ
 Môi trường
Nước pepton đệm
Pepton 10 g

Sodium chloride 5 g
Bile salt 1,5 g
Sorbitol 10 g
Crystal violet 0,001 g
Neutral red 0,003 g
Agar 15 g
Nước cất vừa đủ 1000 ml

SIMMON CITRATE
Na citrate 2 g
NaCl 5 g
K
2
HPO
4
1 g
NH
4
H
2
PO
4
1 g
MgSO
4
0,2 g

43
Bromothymol blue 0,08 g
Agar 15 g

ừng