KHOA
HOC KY THUAT THO
Y
TAP XXI SO 1 - 2014
i0Sl)iln
cifu fefipa
toe
DANH
GlA
HIEU
llfC
CUA
UACXIN
AMERVAC PRRS
001 VOll
CHUNG VIRUS PRRS
GAY
RENH
DONG
llfC
CAO
A
VIET
NAM
To
Long Thanh', Nguyen Hoang Bang', Tdng
HS-u
Hien',
Bach Qudc Thdng', Daniel
Torrent^
TOM TAT

cong cudng
dpe.
Dap ung mien dich co dupc nha vacxin da loai bo hoan toan virus trong ca the
Ipn
v^o
khoang 2-3 tuan sau khi bi nhiem.
Tir
khoa: Lan, PRRS, vacxin PRRS, khao nghiem, cong cuong
dpe
Evaluation of efficiency of Amervac PRRS vaccine for the High virulent,
pathologenic virus strain in Viet Nam
To
Long
Thanh,
Nguyen Hoang
Dang,
Tong
Huu
Hien,
Bach Quoc
Thang,
Daniel Torrents
SUMI\/IARY
Live AMERVAC PRRS vaccine produced by
HIPRA
Laboratory, SA (Spain) was used for
testing on pigs raising in
Viet
Nam The tested result indicated that
this

S.A, Amer Tay Ban Nha
KHOA HOC KY
THUAT
THU
Y TAP XXI
SO
1
- 2014
I.
DAT VAN DE
Hpi chung roi loan sinh san va ho hap
ciia
Ign
(PRRS)
th8
doc luc cao do virus PRRS
(PRRSV)
thuoc genotype II gay ra. The benh
nay xuat
hien
tai Viet Nam nam 2007 va da gay
nen nhung
t6n thSt
kinh
t6
nang ne cho nhirng
nguai chan nuoi lan trong nhiing nam sau do.
Ve lich sir, PRRSV genotype
1
xuat hien lan

2.1.
Nguyfin v3t
lieu
- Dpng vat thi nghiem: Chpn 16 lan con 4
tuan tuoi, khoe manh va am tinh vdi virus PRRS,
khong hem bdt ky mot loai vacxin phong PRRS
nao.
Lan duac nuoi va tien hanh thuc nghiem
tai Trung tam chan doan
thii
y TW.
- Vacxin
nhupc
doc AMERVAC PRRS do
hang Laboratorios HIPRA, S.A san xuat, dung
cho lan 3 tuan
tuoi
tro len.
Virus cuong doc gom 2 chung: Chiing
virus PRRS so 21 do cong ty HIPRA cung cap
CO ngu6n goe
tir Viet Nam nam 2008 va chiing
vims PRRS ky
hieu
VN07196 cua Trung tam
chan doan
thii
y TW.
- Kit
ELlSAphat

HQC K? THUAT THO Y TAP XXI S6 1
-
2014
Theo d5i
Ipn
sau khi
tidm
vacxin
d^
danh gia
tinh an toan, bieu
hi?n
iam sang
\k bi^n
ddi than
nhi?t
tir ngay
diij
32
dgn
46. Can
Ipn,
theo d5i
trpng lupng vao cac ngay thir 0, 32, 39, 36 va
55.
LSy
mau vao cac ngay thu
29,35,
39,42,46
va 55

thS.
- Dimg phuang phap Real-time RT-PCR
d8
kiem tra virus.
- Dung phuang phap
m6
kham va lam
tiSu
bdn vi
thi
de kiem tra
b8nh
tich dai
th8
va vi
thi.
- Phuang phdp ddnh gia an toan
ciia
vacxin:
Theo doi mpt tudn sau khi tiem vacxin, nlu
khong
CO
phdn irng bat
thuong
nhu:
mSt
moi,
chdn
ho^c
b6 dn, tdng than

cuong
dpe
voi cdc chi tieu khac bao
g6m:
Bilu
hien lam sang, tang than nhiet, benh dch dai
the,
vi the a
ph6i,
tinh trang nhilm virus mau va
kiem tra dap irng mien dich bdng
phucmg
phdp
huylt
thanh hoc.
III.
KET
QUA
VA THAO
LUAN
3.1 Ket qua theo doi an
to£kn
sau khi
tiSm
vacxin
Theo doi mot tuan sau khi tiem vacxin 8
Ipn
khdng thay co bat ky cac phan img bdt
thudng
nhu: met moi, chdn hoac bo an, tang than nhiet

J '•''
Q
0.5
—• ^
1
/
X\
/ ^^*^ ^ *\
/
,y^^^
\
"\'"
J
y*
\
^
-^V)t1
-•-DC-1
^ ^ ^ ^ ^ ^ ^ ^ ^ ^ ^ ^ .^ ^ .^
Bieu
do
la.
Bieu
hi^n
tam
sdng
cua
Iqn dirpv
cong virus PRRS 21
KHOA HOC

chap,
it van ddng va gidm dn. Mdt s6 cd trieu
chung thd nang nhpc hoac tim tdi d tai. Co
1
lpn
(s6
11)
d
Id
ddi chung cd bieu hien benh nang
han cac con cdn lai trong id, the trang rat met
mdi,
thd ddc "kieu chd ngdi" vd co hien tupng
tim tai. Ddi vdi nhung lpn
Id
vacxin, cac
tri?u
chung tren khdng bieu hien ro va thudng chi
keo dai 1-2 ngdy, khdng
lau
nhu
Ion
ddi
chumg.
- Tir ngay 8-10 sau khi cdng (ngay thu
40 ttd
di) cac trieu chiing
bSnh
giam ddn, Ion nhanh
nhen ban, an khde. Nhdm Ipn vacxin den ngdy

/—
/,
/
/ \ ,
"\^
\
\
\ \^
V^
\
^\,
\
.^
<^
fij"
4>
4
^
t^
^
t^
1^
' ^^^ ^^^ ^^^ ^^^ ^^^ ^^^ ^^^ ^^"^ ^^^
Bieu do
lb.
Bieu
hi$n
lam sang cua
Ion
dw^

Miic
an d nhimg ngay ndy giam
han so vdi trudc do vd lpn tiem vacxin an nhieu
hon
1pm
ddi chiing. Ld 4 cd 1 Ipn ddi chiing (so
8) cd bieu hien
b8nh
nang horn nhung con cdn
lai trong cung
Id,
thudng nam bep mpt
cho.
Lpn
ndy
chit
vao ngay
thii
43
(tiic
la
11
ngay sau
khi
bi gay nhilm bdng virus PRRS VN07196).
- Lpn
Id
vacxin bdt ddu
hit
cac

2a.
Diin bien than nhiet cua lan
du^
giy
nhi$m
virus
PRRS
21
41.0
O
40.5
1'
40.0
c
S
39.5
1-
39.0
38 5
^/
r-^
r^ y~^~^^^^
It
^~~ 4 ; ,__ _^
J/>
^^'^^—
^^ -^^ /^ /^
4>
4
^^ ^^ ^^ ^^

thii 33 (virus PRRS 21) bic la sau khi cdng
cudng
dpe
1
ngdy.
- Lpn s6t cao nhdt vdo giai doan ngay
thii
35-41
vdi than
nhi$t
trung binh co
liic
len tdi
tren
41''C,
ddc biet la cdc
Ion
d 2
Id
ddi chumg.
Tren bieu dd cho thdy virus PRRS 21 gay sdt
vdi thdi
gian
nhanh hon (sau 3 ngdy, than nhiet
trung binh tren
40°C);
tuy nhien virus
VN07196
lai gay sdt dai han (den ngay
thii

VX-1
BC-1
VX-2
DC-2
Ngay 29
40,00
40,00
40,00
40,00
Ngay 35
29,61
26,69
27,95
26,01
Ngay 39
27,15
24,29
26,93
24,32
Ngay 42
29,98
26,55
28,76
26,20
Ngiy
46
34,99
32,39
33,16
33,75

- C6ng cudng dpe d thdi dilm ngdy
thii
32.
Sau dd lay mdu vao cdc ngdy
thii
35,
39,42,
46
va 55 de kiem tra
mire
dp virus huylt.
Kit
qud
the hien tren bang va bieu do cho thay virus xudt
hien trong huyet thanh lpn dat cao nhdt vdo giai
doan ngay thu 35-42
(tiic
la ngay 3-10 sau khi
gdy nhilm) va giam dan d nhung ngay tiep theo.
- Tai ciing thdi dilm, lupng virus huyet cr
Ion thudc cdc Id ddi chiing cao han Ipn d cdc
16
vacxin tli 4-8 ldn (chenh lech 2-3 Ct).
- Den ngdy
thii
55 khi ket thiic thi
nghi?m,
vdn cdn 1 lupng nhd virus phat hien dupc trong
huyet thanh
Ign

11
12
1
3
18
19
8
14
15
Ngay
0
S/P
0,041943
0,335541
0,311258
0,280353
0,19426
0,328918
0,00662
0,015453
0,07064
0,00662
0,01987
0,011038
0,03532
0,04636
0,02649
0,00662
KQ
Neg

0,0053
0,04278
0,05348
KQ
Pos
Neg
Pos
Pos
Neg
Neg
Neg
Neg
Pos
Neg
Pos
Pos
Neg
Neg
Neg
Neg
Ngay 55
S/P
2,3246
2,09607
2,538574
2,11936
1,41048
1,918486
KQ
Pos

4
Vims cong
PRRS
21
PRI^S
VN 07196
So tai
2
4
5
13
9
10
11
12
1
3
18
19
8
14
15
16
NgayO
Neg
Neg
Neg
Neg
Neg
Neg

1/1280
1/640
1/320
1/640
LON
CHET
1/640
1/640
1/320
1/1280
1/320
LgN CH^T
1/640
1/320
1/320
KHOA HOC
KY THUAT
THU
Y TAP XXI SO 1
- 2014
Nhdn xet:
- Trudc khi tiem vacxin vdo ngay 0, tat cd
16
Ion ciia
4
Id
deu dm tinh vai khang the khang
virus PRRS (ca 2 phuang phdp xet nghiem
ELISA va
IPMA)

sdng sdt (mdi 16 d6i chiing chet 1/4 lpn). Ty
1^
S/P trung binh vd
hi$u
gia khang the trung binh
d 2
Id
tiem vacxin cao hon 2
Id
ddi chiing Co
the nhdn thdy su chuyen
biSn
ddp
iing
miln dich
tao khdng the thdng qua cac ket qua ciia phuang
phdp ELISA va IPMA sau khi tiem vacxin va
sau khi cdng cudng ddc.
Dilu nay chung to
vi|c sir
dung vacxin
da
kich
thich hinh thdnh dap
ung
miln dich
ddc
hi§u.
Ddp
iing

5,9
6,3
6,2
Ngay 32
(CCB)
14,4
14,3
14,5
14,6
Ngay 39
16,5
14,8
16,9
15,2
Ngay 46
19,2
16,1
19,8
16,6
Ngiy 55
22,8
19,0
23,5
19,6
-
O
giai doan sau khi
tiem
vacxin (ngay 0
den ngdy 32),

4,1
kg.
L6
dung vacxin AMERVAC PRRS
tSng
trong tdt
h<ra
so vdi
15
khong dung vacxin khi
cong virus PRRS
cu-dng
dOc.
Dilu
dd
chung
td vacxin bao hp
Ign Qaqic
tiem trdnh nhirng
tac hai cua virus PRRS
cu-dng
dpe.
KHOA HOC KY THUAT THU Y
TAP
XXI SO 1
-
2014
3.2.5.
Bfnh
tich

2+
So
3
2+
So
18
2+
So
19
1 +
Diem trung
binii
io = 1,75
mo Itham
Lo 2 - doi
chijng
So
9
3->
So 10
2+
s6ii
3+
S6l2
2+
Diem
tnjng
binh io = 2,50
Lo 4 - d6i
clii>ng

vd xudt huyet, xudt hien ca d
Id
vacxin va
Id
ddi chiing. Benh tich dai
th€ o
phdi ciia Ign
Id tiem vacxin cd bieu hien viem
nhg hon
so
v^
Ign cua
Id
doi chirng.
Ld 2 - Doi
chLPng
cong PRRS 21
PhSl viem
ke,
xuit
huyil Hach
khi
phe quan
sung,
xuit
huySt
Lo
1
-Vacxin cong PRRS 21
1

21)
vd
Id
4
(cdng
vims PRRS
VN
07196)
cd benh tich nang
phii
ne mang phdi,
vdch ngan gian rpng, Idng phe quan chiia dich
ri viem va tl bdo viem, hinh thanh huyet
khdi,
viem mach tang sinh, vach
phi
nang day tang
sinh te bao
phi
nang loai 2. Lo 2 cd bieu hien
benh tich ndng ne
hem 16
4.
- Benh tich vi the cdc
16
vx: L6
1 (cdng
virus
PRRS
21)

thg;
Lo 2
B/C
(cdng virus PRRS 21)
:~'-i5.^
V<;;
\
%,
Phdi: Phe quan
chCra
dich viem, bong troc
te bao biiu mo; vach
ph§
nang day, tham
nhiSm
cac
tS
bao viem
Hach khi phe quan: Nang lympho gian
rong, co nhidu t4 bao lympho dang hoat
dong va dai thirc bao
Lo 1 VX (cong virus PRRS 21)
Phdi: Vach ngan day, c6 nhiSu te bao
viem xam nh$p: vach phe nang day
Hach khi phe quan: Nang lympho gian
rong, tang sinh dai thirc bao
Lo 4
Die
(cong virus PRRS -VN 07196)
Ph6i:

thich sinh dap ung mien dich khi Ian bi nhiem
virus cudng ddc (hodc c6ng cudng doc) dap ung
mien dich se manh ban.
- Dap ling mien dich do vacxin tao ra cd tac
dung 1dm giam
miic
dp tram trpng cua benh
thdng qua kiem tra benh tich dai the vd vi the
tren cac ca quan npi tang, dac biet la ph6i cua
lpn sau khi bi nhiem virus cudng doc PRRS.
- Lo diing vacxin AMERVAC PRRS tang
trpng ban so vdi
Id
khdng dimg vacxin khi cdng
virus PRRS cuong ddc.
- Dap ling mien dich co dupc nhd vacxin
da loai bd hoan todn virus trong ca the Ipn vao
khodng 2-3 tudn sau khi bi nhilm. Lpn
dugc
tiem vacxin, khi bi nhiem virut cudng doc se
khdng dao thai virus cudng ddc ra mdi
tnidng
xung quanh.
TAI
LIEU
THAM KHAO
1.
Tian et al. 2007.
PloS
ONE 2: e526.